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Binding of interferon reduces the force of unfolding for interferon receptor 1
Differential signaling of the type I interferon receptor (IFNAR) has been correlated with the ability of its subunit, IFNAR1, to differentially recognize a large spectrum of different ligands, which involves intricate conformational re-arrangements of multiple interacting domains. To shed light onto...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5389645/ https://www.ncbi.nlm.nih.gov/pubmed/28403186 http://dx.doi.org/10.1371/journal.pone.0175413 |
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author | Chuartzman, Silvia G. Nevo, Reinat Waichman, Sharon Shental, Dalit Piehler, Jacob Levy, Yaakov Reich, Ziv Kapon, Ruti |
author_facet | Chuartzman, Silvia G. Nevo, Reinat Waichman, Sharon Shental, Dalit Piehler, Jacob Levy, Yaakov Reich, Ziv Kapon, Ruti |
author_sort | Chuartzman, Silvia G. |
collection | PubMed |
description | Differential signaling of the type I interferon receptor (IFNAR) has been correlated with the ability of its subunit, IFNAR1, to differentially recognize a large spectrum of different ligands, which involves intricate conformational re-arrangements of multiple interacting domains. To shed light onto the structural determinants governing ligand recognition, we compared the force-induced unfolding of the IFNAR1 ectodomain when bound to interferon and when free, using the atomic force microscope and steered molecular dynamics simulations. Unexpectedly, we find that IFNAR1 is easier to mechanically unfold when bound to interferon than when free. Analysis of the structures indicated that the origin of the reduction in unfolding forces is a conformational change in IFNAR1 induced by ligand binding. |
format | Online Article Text |
id | pubmed-5389645 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-53896452017-05-03 Binding of interferon reduces the force of unfolding for interferon receptor 1 Chuartzman, Silvia G. Nevo, Reinat Waichman, Sharon Shental, Dalit Piehler, Jacob Levy, Yaakov Reich, Ziv Kapon, Ruti PLoS One Research Article Differential signaling of the type I interferon receptor (IFNAR) has been correlated with the ability of its subunit, IFNAR1, to differentially recognize a large spectrum of different ligands, which involves intricate conformational re-arrangements of multiple interacting domains. To shed light onto the structural determinants governing ligand recognition, we compared the force-induced unfolding of the IFNAR1 ectodomain when bound to interferon and when free, using the atomic force microscope and steered molecular dynamics simulations. Unexpectedly, we find that IFNAR1 is easier to mechanically unfold when bound to interferon than when free. Analysis of the structures indicated that the origin of the reduction in unfolding forces is a conformational change in IFNAR1 induced by ligand binding. Public Library of Science 2017-04-12 /pmc/articles/PMC5389645/ /pubmed/28403186 http://dx.doi.org/10.1371/journal.pone.0175413 Text en © 2017 Chuartzman et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Chuartzman, Silvia G. Nevo, Reinat Waichman, Sharon Shental, Dalit Piehler, Jacob Levy, Yaakov Reich, Ziv Kapon, Ruti Binding of interferon reduces the force of unfolding for interferon receptor 1 |
title | Binding of interferon reduces the force of unfolding for interferon receptor 1 |
title_full | Binding of interferon reduces the force of unfolding for interferon receptor 1 |
title_fullStr | Binding of interferon reduces the force of unfolding for interferon receptor 1 |
title_full_unstemmed | Binding of interferon reduces the force of unfolding for interferon receptor 1 |
title_short | Binding of interferon reduces the force of unfolding for interferon receptor 1 |
title_sort | binding of interferon reduces the force of unfolding for interferon receptor 1 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5389645/ https://www.ncbi.nlm.nih.gov/pubmed/28403186 http://dx.doi.org/10.1371/journal.pone.0175413 |
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