Direct fluorescence detection of VirE2 secretion by Agrobacterium tumefaciens

VirE2 is a ssDNA binding protein essential for virulence in Agrobacterium tumefaciens. A tetracysteine mutant (VirE2-TC) was prepared for in vitro and in vivo fluorescence imaging based on the ReAsH reagent. VirE2-TC was found to be biochemically active as it binds both ssDNA and the acidic secretio...

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Detalles Bibliográficos
Autores principales: Yaakov, Noga, Barak, Yoav, Pereman, Idan, Christie, Peter J., Elbaum, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5389803/
https://www.ncbi.nlm.nih.gov/pubmed/28403156
http://dx.doi.org/10.1371/journal.pone.0175273

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5389803/
https://www.ncbi.nlm.nih.gov/pubmed/28403156
http://dx.doi.org/10.1371/journal.pone.0175273

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