Amplification of a transgene within a long array of replication origins favors higher gene expression in animal cells

Plasmids with both a mammalian replication initiation region (IR) and a matrix attachment region (MAR) are spontaneously amplified in transfected cells, and generate extrachromosomal double minute (DM) or chromosomal homogeneously staining region (HSR). We previously isolated the shortest core IR (G...

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Detalles Bibliográficos
Autores principales: Ohsaki, Kiwamu, Ohgaki, Yusuke, Shimizu, Noriaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5389822/
https://www.ncbi.nlm.nih.gov/pubmed/28403180
http://dx.doi.org/10.1371/journal.pone.0175585

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5389822/
https://www.ncbi.nlm.nih.gov/pubmed/28403180
http://dx.doi.org/10.1371/journal.pone.0175585

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