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Amplification of a transgene within a long array of replication origins favors higher gene expression in animal cells
Plasmids with both a mammalian replication initiation region (IR) and a matrix attachment region (MAR) are spontaneously amplified in transfected cells, and generate extrachromosomal double minute (DM) or chromosomal homogeneously staining region (HSR). We previously isolated the shortest core IR (G...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5389822/ https://www.ncbi.nlm.nih.gov/pubmed/28403180 http://dx.doi.org/10.1371/journal.pone.0175585 |