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Identification of proteins involved in the anti-inflammatory properties of Propionibacterium freudenreichii by means of a multi-strain study
Propionibacterium freudenreichii, a dairy starter, can reach a population of almost 10(9) propionibacteria per gram in Swiss-type cheese at the time of consumption. Also consumed as a probiotic, it displays strain-dependent anti-inflammatory properties mediated by surface proteins that induce IL-10...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5390290/ https://www.ncbi.nlm.nih.gov/pubmed/28406170 http://dx.doi.org/10.1038/srep46409 |
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author | Deutsch, Stéphanie-Marie Mariadassou, Mahendra Nicolas, Pierre Parayre, Sandrine Le Guellec, Rozenn Chuat, Victoria Peton, Vincent Le Maréchal, Caroline Burati, Julien Loux, Valentin Briard-Bion, Valérie Jardin, Julien Plé, Coline Foligné, Benoît Jan, Gwénaël Falentin, Hélène |
author_facet | Deutsch, Stéphanie-Marie Mariadassou, Mahendra Nicolas, Pierre Parayre, Sandrine Le Guellec, Rozenn Chuat, Victoria Peton, Vincent Le Maréchal, Caroline Burati, Julien Loux, Valentin Briard-Bion, Valérie Jardin, Julien Plé, Coline Foligné, Benoît Jan, Gwénaël Falentin, Hélène |
author_sort | Deutsch, Stéphanie-Marie |
collection | PubMed |
description | Propionibacterium freudenreichii, a dairy starter, can reach a population of almost 10(9) propionibacteria per gram in Swiss-type cheese at the time of consumption. Also consumed as a probiotic, it displays strain-dependent anti-inflammatory properties mediated by surface proteins that induce IL-10 in leukocytes. We selected 23 strains with varied anti-inflammatory potentials in order to identify the protein(s) involved. After comparative genomic analysis, 12 of these strains were further analysed by surface proteomics, eight of them being further submitted to transcriptomics. The omics data were then correlated to the anti-inflammatory potential evaluated by IL-10 induction. This comparative omics strategy highlighted candidate genes that were further subjected to gene-inactivation validation. This validation confirmed the contribution of surface proteins, including SlpB and SlpE, two proteins with SLH domains known to mediate non-covalent anchorage to the cell-wall. Interestingly, HsdM3, predicted as cytoplasmic and involved in DNA modification, was shown to contribute to anti-inflammatory activity. Finally, we demonstrated that a single protein cannot explain the anti-inflammatory properties of a strain. These properties therefore result from different combinations of surface and cytoplasmic proteins, depending on the strain. Our enhanced understanding of the molecular bases for immunomodulation will enable the relevant screening for bacterial resources with anti-inflammatory properties. |
format | Online Article Text |
id | pubmed-5390290 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-53902902017-04-14 Identification of proteins involved in the anti-inflammatory properties of Propionibacterium freudenreichii by means of a multi-strain study Deutsch, Stéphanie-Marie Mariadassou, Mahendra Nicolas, Pierre Parayre, Sandrine Le Guellec, Rozenn Chuat, Victoria Peton, Vincent Le Maréchal, Caroline Burati, Julien Loux, Valentin Briard-Bion, Valérie Jardin, Julien Plé, Coline Foligné, Benoît Jan, Gwénaël Falentin, Hélène Sci Rep Article Propionibacterium freudenreichii, a dairy starter, can reach a population of almost 10(9) propionibacteria per gram in Swiss-type cheese at the time of consumption. Also consumed as a probiotic, it displays strain-dependent anti-inflammatory properties mediated by surface proteins that induce IL-10 in leukocytes. We selected 23 strains with varied anti-inflammatory potentials in order to identify the protein(s) involved. After comparative genomic analysis, 12 of these strains were further analysed by surface proteomics, eight of them being further submitted to transcriptomics. The omics data were then correlated to the anti-inflammatory potential evaluated by IL-10 induction. This comparative omics strategy highlighted candidate genes that were further subjected to gene-inactivation validation. This validation confirmed the contribution of surface proteins, including SlpB and SlpE, two proteins with SLH domains known to mediate non-covalent anchorage to the cell-wall. Interestingly, HsdM3, predicted as cytoplasmic and involved in DNA modification, was shown to contribute to anti-inflammatory activity. Finally, we demonstrated that a single protein cannot explain the anti-inflammatory properties of a strain. These properties therefore result from different combinations of surface and cytoplasmic proteins, depending on the strain. Our enhanced understanding of the molecular bases for immunomodulation will enable the relevant screening for bacterial resources with anti-inflammatory properties. Nature Publishing Group 2017-04-13 /pmc/articles/PMC5390290/ /pubmed/28406170 http://dx.doi.org/10.1038/srep46409 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Deutsch, Stéphanie-Marie Mariadassou, Mahendra Nicolas, Pierre Parayre, Sandrine Le Guellec, Rozenn Chuat, Victoria Peton, Vincent Le Maréchal, Caroline Burati, Julien Loux, Valentin Briard-Bion, Valérie Jardin, Julien Plé, Coline Foligné, Benoît Jan, Gwénaël Falentin, Hélène Identification of proteins involved in the anti-inflammatory properties of Propionibacterium freudenreichii by means of a multi-strain study |
title | Identification of proteins involved in the anti-inflammatory properties of Propionibacterium freudenreichii by means of a multi-strain study |
title_full | Identification of proteins involved in the anti-inflammatory properties of Propionibacterium freudenreichii by means of a multi-strain study |
title_fullStr | Identification of proteins involved in the anti-inflammatory properties of Propionibacterium freudenreichii by means of a multi-strain study |
title_full_unstemmed | Identification of proteins involved in the anti-inflammatory properties of Propionibacterium freudenreichii by means of a multi-strain study |
title_short | Identification of proteins involved in the anti-inflammatory properties of Propionibacterium freudenreichii by means of a multi-strain study |
title_sort | identification of proteins involved in the anti-inflammatory properties of propionibacterium freudenreichii by means of a multi-strain study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5390290/ https://www.ncbi.nlm.nih.gov/pubmed/28406170 http://dx.doi.org/10.1038/srep46409 |
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