Cargando…

Identification of proteins involved in the anti-inflammatory properties of Propionibacterium freudenreichii by means of a multi-strain study

Propionibacterium freudenreichii, a dairy starter, can reach a population of almost 10(9) propionibacteria per gram in Swiss-type cheese at the time of consumption. Also consumed as a probiotic, it displays strain-dependent anti-inflammatory properties mediated by surface proteins that induce IL-10...

Descripción completa

Detalles Bibliográficos
Autores principales: Deutsch, Stéphanie-Marie, Mariadassou, Mahendra, Nicolas, Pierre, Parayre, Sandrine, Le Guellec, Rozenn, Chuat, Victoria, Peton, Vincent, Le Maréchal, Caroline, Burati, Julien, Loux, Valentin, Briard-Bion, Valérie, Jardin, Julien, Plé, Coline, Foligné, Benoît, Jan, Gwénaël, Falentin, Hélène
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5390290/
https://www.ncbi.nlm.nih.gov/pubmed/28406170
http://dx.doi.org/10.1038/srep46409
_version_ 1782521429064941568
author Deutsch, Stéphanie-Marie
Mariadassou, Mahendra
Nicolas, Pierre
Parayre, Sandrine
Le Guellec, Rozenn
Chuat, Victoria
Peton, Vincent
Le Maréchal, Caroline
Burati, Julien
Loux, Valentin
Briard-Bion, Valérie
Jardin, Julien
Plé, Coline
Foligné, Benoît
Jan, Gwénaël
Falentin, Hélène
author_facet Deutsch, Stéphanie-Marie
Mariadassou, Mahendra
Nicolas, Pierre
Parayre, Sandrine
Le Guellec, Rozenn
Chuat, Victoria
Peton, Vincent
Le Maréchal, Caroline
Burati, Julien
Loux, Valentin
Briard-Bion, Valérie
Jardin, Julien
Plé, Coline
Foligné, Benoît
Jan, Gwénaël
Falentin, Hélène
author_sort Deutsch, Stéphanie-Marie
collection PubMed
description Propionibacterium freudenreichii, a dairy starter, can reach a population of almost 10(9) propionibacteria per gram in Swiss-type cheese at the time of consumption. Also consumed as a probiotic, it displays strain-dependent anti-inflammatory properties mediated by surface proteins that induce IL-10 in leukocytes. We selected 23 strains with varied anti-inflammatory potentials in order to identify the protein(s) involved. After comparative genomic analysis, 12 of these strains were further analysed by surface proteomics, eight of them being further submitted to transcriptomics. The omics data were then correlated to the anti-inflammatory potential evaluated by IL-10 induction. This comparative omics strategy highlighted candidate genes that were further subjected to gene-inactivation validation. This validation confirmed the contribution of surface proteins, including SlpB and SlpE, two proteins with SLH domains known to mediate non-covalent anchorage to the cell-wall. Interestingly, HsdM3, predicted as cytoplasmic and involved in DNA modification, was shown to contribute to anti-inflammatory activity. Finally, we demonstrated that a single protein cannot explain the anti-inflammatory properties of a strain. These properties therefore result from different combinations of surface and cytoplasmic proteins, depending on the strain. Our enhanced understanding of the molecular bases for immunomodulation will enable the relevant screening for bacterial resources with anti-inflammatory properties.
format Online
Article
Text
id pubmed-5390290
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-53902902017-04-14 Identification of proteins involved in the anti-inflammatory properties of Propionibacterium freudenreichii by means of a multi-strain study Deutsch, Stéphanie-Marie Mariadassou, Mahendra Nicolas, Pierre Parayre, Sandrine Le Guellec, Rozenn Chuat, Victoria Peton, Vincent Le Maréchal, Caroline Burati, Julien Loux, Valentin Briard-Bion, Valérie Jardin, Julien Plé, Coline Foligné, Benoît Jan, Gwénaël Falentin, Hélène Sci Rep Article Propionibacterium freudenreichii, a dairy starter, can reach a population of almost 10(9) propionibacteria per gram in Swiss-type cheese at the time of consumption. Also consumed as a probiotic, it displays strain-dependent anti-inflammatory properties mediated by surface proteins that induce IL-10 in leukocytes. We selected 23 strains with varied anti-inflammatory potentials in order to identify the protein(s) involved. After comparative genomic analysis, 12 of these strains were further analysed by surface proteomics, eight of them being further submitted to transcriptomics. The omics data were then correlated to the anti-inflammatory potential evaluated by IL-10 induction. This comparative omics strategy highlighted candidate genes that were further subjected to gene-inactivation validation. This validation confirmed the contribution of surface proteins, including SlpB and SlpE, two proteins with SLH domains known to mediate non-covalent anchorage to the cell-wall. Interestingly, HsdM3, predicted as cytoplasmic and involved in DNA modification, was shown to contribute to anti-inflammatory activity. Finally, we demonstrated that a single protein cannot explain the anti-inflammatory properties of a strain. These properties therefore result from different combinations of surface and cytoplasmic proteins, depending on the strain. Our enhanced understanding of the molecular bases for immunomodulation will enable the relevant screening for bacterial resources with anti-inflammatory properties. Nature Publishing Group 2017-04-13 /pmc/articles/PMC5390290/ /pubmed/28406170 http://dx.doi.org/10.1038/srep46409 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Deutsch, Stéphanie-Marie
Mariadassou, Mahendra
Nicolas, Pierre
Parayre, Sandrine
Le Guellec, Rozenn
Chuat, Victoria
Peton, Vincent
Le Maréchal, Caroline
Burati, Julien
Loux, Valentin
Briard-Bion, Valérie
Jardin, Julien
Plé, Coline
Foligné, Benoît
Jan, Gwénaël
Falentin, Hélène
Identification of proteins involved in the anti-inflammatory properties of Propionibacterium freudenreichii by means of a multi-strain study
title Identification of proteins involved in the anti-inflammatory properties of Propionibacterium freudenreichii by means of a multi-strain study
title_full Identification of proteins involved in the anti-inflammatory properties of Propionibacterium freudenreichii by means of a multi-strain study
title_fullStr Identification of proteins involved in the anti-inflammatory properties of Propionibacterium freudenreichii by means of a multi-strain study
title_full_unstemmed Identification of proteins involved in the anti-inflammatory properties of Propionibacterium freudenreichii by means of a multi-strain study
title_short Identification of proteins involved in the anti-inflammatory properties of Propionibacterium freudenreichii by means of a multi-strain study
title_sort identification of proteins involved in the anti-inflammatory properties of propionibacterium freudenreichii by means of a multi-strain study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5390290/
https://www.ncbi.nlm.nih.gov/pubmed/28406170
http://dx.doi.org/10.1038/srep46409
work_keys_str_mv AT deutschstephaniemarie identificationofproteinsinvolvedintheantiinflammatorypropertiesofpropionibacteriumfreudenreichiibymeansofamultistrainstudy
AT mariadassoumahendra identificationofproteinsinvolvedintheantiinflammatorypropertiesofpropionibacteriumfreudenreichiibymeansofamultistrainstudy
AT nicolaspierre identificationofproteinsinvolvedintheantiinflammatorypropertiesofpropionibacteriumfreudenreichiibymeansofamultistrainstudy
AT parayresandrine identificationofproteinsinvolvedintheantiinflammatorypropertiesofpropionibacteriumfreudenreichiibymeansofamultistrainstudy
AT leguellecrozenn identificationofproteinsinvolvedintheantiinflammatorypropertiesofpropionibacteriumfreudenreichiibymeansofamultistrainstudy
AT chuatvictoria identificationofproteinsinvolvedintheantiinflammatorypropertiesofpropionibacteriumfreudenreichiibymeansofamultistrainstudy
AT petonvincent identificationofproteinsinvolvedintheantiinflammatorypropertiesofpropionibacteriumfreudenreichiibymeansofamultistrainstudy
AT lemarechalcaroline identificationofproteinsinvolvedintheantiinflammatorypropertiesofpropionibacteriumfreudenreichiibymeansofamultistrainstudy
AT buratijulien identificationofproteinsinvolvedintheantiinflammatorypropertiesofpropionibacteriumfreudenreichiibymeansofamultistrainstudy
AT louxvalentin identificationofproteinsinvolvedintheantiinflammatorypropertiesofpropionibacteriumfreudenreichiibymeansofamultistrainstudy
AT briardbionvalerie identificationofproteinsinvolvedintheantiinflammatorypropertiesofpropionibacteriumfreudenreichiibymeansofamultistrainstudy
AT jardinjulien identificationofproteinsinvolvedintheantiinflammatorypropertiesofpropionibacteriumfreudenreichiibymeansofamultistrainstudy
AT plecoline identificationofproteinsinvolvedintheantiinflammatorypropertiesofpropionibacteriumfreudenreichiibymeansofamultistrainstudy
AT folignebenoit identificationofproteinsinvolvedintheantiinflammatorypropertiesofpropionibacteriumfreudenreichiibymeansofamultistrainstudy
AT jangwenael identificationofproteinsinvolvedintheantiinflammatorypropertiesofpropionibacteriumfreudenreichiibymeansofamultistrainstudy
AT falentinhelene identificationofproteinsinvolvedintheantiinflammatorypropertiesofpropionibacteriumfreudenreichiibymeansofamultistrainstudy