QuickLib, a method for building fully synthetic plasmid libraries by seamless cloning of degenerate oligonucleotides

Incorporation of synthetic degenerate oligonucleotides into plasmids for building highly diverse genetic libraries requires efficient and quantitative DNA manipulation. We present a fast and seamless method for generating libraries of PCR-synthesized plasmids designed with a degenerate sequence and...

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Detalles Bibliográficos
Autores principales: Galka, Pierre, Jamez, Elisabeth, Joachim, Gilles, Soumillion, Patrice
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5390991/
https://www.ncbi.nlm.nih.gov/pubmed/28406948
http://dx.doi.org/10.1371/journal.pone.0175146

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5390991/
https://www.ncbi.nlm.nih.gov/pubmed/28406948
http://dx.doi.org/10.1371/journal.pone.0175146

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