QuickLib, a method for building fully synthetic plasmid libraries by seamless cloning of degenerate oligonucleotides

Incorporation of synthetic degenerate oligonucleotides into plasmids for building highly diverse genetic libraries requires efficient and quantitative DNA manipulation. We present a fast and seamless method for generating libraries of PCR-synthesized plasmids designed with a degenerate sequence and...

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Detalles Bibliográficos
Autores principales: Galka, Pierre, Jamez, Elisabeth, Joachim, Gilles, Soumillion, Patrice
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5390991/
https://www.ncbi.nlm.nih.gov/pubmed/28406948
http://dx.doi.org/10.1371/journal.pone.0175146
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author Galka, Pierre
Jamez, Elisabeth
Joachim, Gilles
Soumillion, Patrice
author_facet Galka, Pierre
Jamez, Elisabeth
Joachim, Gilles
Soumillion, Patrice
author_sort Galka, Pierre
collection PubMed
description Incorporation of synthetic degenerate oligonucleotides into plasmids for building highly diverse genetic libraries requires efficient and quantitative DNA manipulation. We present a fast and seamless method for generating libraries of PCR-synthesized plasmids designed with a degenerate sequence and short overlapping ends. Our method called QuickLib should find many applications in synthetic biology; as an example, we easily prepared genetic libraries of Escherichia coli expressing billions of different backbone cyclic peptides.
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spelling pubmed-53909912017-05-03 QuickLib, a method for building fully synthetic plasmid libraries by seamless cloning of degenerate oligonucleotides Galka, Pierre Jamez, Elisabeth Joachim, Gilles Soumillion, Patrice PLoS One Research Article Incorporation of synthetic degenerate oligonucleotides into plasmids for building highly diverse genetic libraries requires efficient and quantitative DNA manipulation. We present a fast and seamless method for generating libraries of PCR-synthesized plasmids designed with a degenerate sequence and short overlapping ends. Our method called QuickLib should find many applications in synthetic biology; as an example, we easily prepared genetic libraries of Escherichia coli expressing billions of different backbone cyclic peptides. Public Library of Science 2017-04-13 /pmc/articles/PMC5390991/ /pubmed/28406948 http://dx.doi.org/10.1371/journal.pone.0175146 Text en © 2017 Galka et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Galka, Pierre
Jamez, Elisabeth
Joachim, Gilles
Soumillion, Patrice
QuickLib, a method for building fully synthetic plasmid libraries by seamless cloning of degenerate oligonucleotides
title QuickLib, a method for building fully synthetic plasmid libraries by seamless cloning of degenerate oligonucleotides
title_full QuickLib, a method for building fully synthetic plasmid libraries by seamless cloning of degenerate oligonucleotides
title_fullStr QuickLib, a method for building fully synthetic plasmid libraries by seamless cloning of degenerate oligonucleotides
title_full_unstemmed QuickLib, a method for building fully synthetic plasmid libraries by seamless cloning of degenerate oligonucleotides
title_short QuickLib, a method for building fully synthetic plasmid libraries by seamless cloning of degenerate oligonucleotides
title_sort quicklib, a method for building fully synthetic plasmid libraries by seamless cloning of degenerate oligonucleotides
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5390991/
https://www.ncbi.nlm.nih.gov/pubmed/28406948
http://dx.doi.org/10.1371/journal.pone.0175146
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