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Dual functionality of cis-regulatory elements as developmental enhancers and Polycomb response elements

Developmental gene expression is tightly regulated through enhancer elements, which initiate dynamic spatio–temporal expression, and Polycomb response elements (PREs), which maintain stable gene silencing. These two cis-regulatory functions are thought to operate through distinct dedicated elements....

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Autores principales: Erceg, Jelena, Pakozdi, Tibor, Marco-Ferreres, Raquel, Ghavi-Helm, Yad, Girardot, Charles, Bracken, Adrian P., Furlong, Eileen E.M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5393054/
https://www.ncbi.nlm.nih.gov/pubmed/28381411
http://dx.doi.org/10.1101/gad.292870.116
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author Erceg, Jelena
Pakozdi, Tibor
Marco-Ferreres, Raquel
Ghavi-Helm, Yad
Girardot, Charles
Bracken, Adrian P.
Furlong, Eileen E.M.
author_facet Erceg, Jelena
Pakozdi, Tibor
Marco-Ferreres, Raquel
Ghavi-Helm, Yad
Girardot, Charles
Bracken, Adrian P.
Furlong, Eileen E.M.
author_sort Erceg, Jelena
collection PubMed
description Developmental gene expression is tightly regulated through enhancer elements, which initiate dynamic spatio–temporal expression, and Polycomb response elements (PREs), which maintain stable gene silencing. These two cis-regulatory functions are thought to operate through distinct dedicated elements. By examining the occupancy of the Drosophila pleiohomeotic repressive complex (PhoRC) during embryogenesis, we revealed extensive co-occupancy at developmental enhancers. Using an established in vivo assay for PRE activity, we demonstrated that a subset of characterized developmental enhancers can function as PREs, silencing transcription in a Polycomb-dependent manner. Conversely, some classic Drosophila PREs can function as developmental enhancers in vivo, activating spatio–temporal expression. This study therefore uncovers elements with dual function: activating transcription in some cells (enhancers) while stably maintaining transcriptional silencing in others (PREs). Given that enhancers initiate spatio–temporal gene expression, reuse of the same elements by the Polycomb group (PcG) system may help fine-tune gene expression and ensure the timely maintenance of cell identities.
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spelling pubmed-53930542017-05-01 Dual functionality of cis-regulatory elements as developmental enhancers and Polycomb response elements Erceg, Jelena Pakozdi, Tibor Marco-Ferreres, Raquel Ghavi-Helm, Yad Girardot, Charles Bracken, Adrian P. Furlong, Eileen E.M. Genes Dev Research Paper Developmental gene expression is tightly regulated through enhancer elements, which initiate dynamic spatio–temporal expression, and Polycomb response elements (PREs), which maintain stable gene silencing. These two cis-regulatory functions are thought to operate through distinct dedicated elements. By examining the occupancy of the Drosophila pleiohomeotic repressive complex (PhoRC) during embryogenesis, we revealed extensive co-occupancy at developmental enhancers. Using an established in vivo assay for PRE activity, we demonstrated that a subset of characterized developmental enhancers can function as PREs, silencing transcription in a Polycomb-dependent manner. Conversely, some classic Drosophila PREs can function as developmental enhancers in vivo, activating spatio–temporal expression. This study therefore uncovers elements with dual function: activating transcription in some cells (enhancers) while stably maintaining transcriptional silencing in others (PREs). Given that enhancers initiate spatio–temporal gene expression, reuse of the same elements by the Polycomb group (PcG) system may help fine-tune gene expression and ensure the timely maintenance of cell identities. Cold Spring Harbor Laboratory Press 2017-03-15 /pmc/articles/PMC5393054/ /pubmed/28381411 http://dx.doi.org/10.1101/gad.292870.116 Text en © 2017 Erceg et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by/4.0/ This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Paper
Erceg, Jelena
Pakozdi, Tibor
Marco-Ferreres, Raquel
Ghavi-Helm, Yad
Girardot, Charles
Bracken, Adrian P.
Furlong, Eileen E.M.
Dual functionality of cis-regulatory elements as developmental enhancers and Polycomb response elements
title Dual functionality of cis-regulatory elements as developmental enhancers and Polycomb response elements
title_full Dual functionality of cis-regulatory elements as developmental enhancers and Polycomb response elements
title_fullStr Dual functionality of cis-regulatory elements as developmental enhancers and Polycomb response elements
title_full_unstemmed Dual functionality of cis-regulatory elements as developmental enhancers and Polycomb response elements
title_short Dual functionality of cis-regulatory elements as developmental enhancers and Polycomb response elements
title_sort dual functionality of cis-regulatory elements as developmental enhancers and polycomb response elements
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5393054/
https://www.ncbi.nlm.nih.gov/pubmed/28381411
http://dx.doi.org/10.1101/gad.292870.116
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