Efficient size-independent chromosome delivery from yeast to cultured cell lines

The delivery of large DNA vectors (>100 000 bp) remains a limiting step in the engineering of mammalian cells and the development of human artificial chromosomes (HACs). Yeast is commonly used to assemble genetic constructs in the megabase size range, and has previously been used to transfer cons...

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Detalles Bibliográficos
Autores principales: Brown, David M., Chan, Yujia A., Desai, Prashant J., Grzesik, Peter, Oldfield, Lauren M., Vashee, Sanjay, Way, Jeffrey C., Silver, Pamela A., Glass, John I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5397165/
https://www.ncbi.nlm.nih.gov/pubmed/27980064
http://dx.doi.org/10.1093/nar/gkw1252

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5397165/
https://www.ncbi.nlm.nih.gov/pubmed/27980064
http://dx.doi.org/10.1093/nar/gkw1252

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