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Cell cycle profiling by image and flow cytometry: The optimised protocol for the detection of replicational activity using 5-Bromo-2′-deoxyuridine, low concentration of hydrochloric acid and exonuclease III

The approach for the detection of replicational activity in cells using 5-bromo-2′-deoxyuridine, a low concentration of hydrochloric acid and exonuclease III is presented in the study. The described method was optimised with the aim to provide a fast and robust tool for the detection of DNA synthesi...

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Detalles Bibliográficos
Autores principales: Ligasová, Anna, Konečný, Petr, Frydrych, Ivo, Koberna, Karel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5398562/
https://www.ncbi.nlm.nih.gov/pubmed/28426799
http://dx.doi.org/10.1371/journal.pone.0175880