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Enabling the controlled assembly of antibody conjugates with a loading of two modules without antibody engineering

The generation of antibody conjugates with a loading of two modules is desirable for a host of reasons. Whilst certain antibody engineering approaches have been useful in the preparation of such constructs, a reliable method based on a native antibody scaffold without the use of enzymes or harsh oxi...

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Autores principales: Lee, Maximillian T. W., Maruani, Antoine, Richards, Daniel A., Baker, James R., Caddick, Stephen, Chudasama, Vijay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5399535/
https://www.ncbi.nlm.nih.gov/pubmed/28451324
http://dx.doi.org/10.1039/c6sc03655d
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author Lee, Maximillian T. W.
Maruani, Antoine
Richards, Daniel A.
Baker, James R.
Caddick, Stephen
Chudasama, Vijay
author_facet Lee, Maximillian T. W.
Maruani, Antoine
Richards, Daniel A.
Baker, James R.
Caddick, Stephen
Chudasama, Vijay
author_sort Lee, Maximillian T. W.
collection PubMed
description The generation of antibody conjugates with a loading of two modules is desirable for a host of reasons. Whilst certain antibody engineering approaches have been useful in the preparation of such constructs, a reliable method based on a native antibody scaffold without the use of enzymes or harsh oxidative conditions has hitherto not been achieved. The use of native antibodies has several advantages in terms of cost, practicality, accessibility, time and overall efficiency. Herein we present a novel, reliable method of furnishing antibody conjugates with a loading of two modules starting from a native antibody scaffold.
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spelling pubmed-53995352017-04-27 Enabling the controlled assembly of antibody conjugates with a loading of two modules without antibody engineering Lee, Maximillian T. W. Maruani, Antoine Richards, Daniel A. Baker, James R. Caddick, Stephen Chudasama, Vijay Chem Sci Chemistry The generation of antibody conjugates with a loading of two modules is desirable for a host of reasons. Whilst certain antibody engineering approaches have been useful in the preparation of such constructs, a reliable method based on a native antibody scaffold without the use of enzymes or harsh oxidative conditions has hitherto not been achieved. The use of native antibodies has several advantages in terms of cost, practicality, accessibility, time and overall efficiency. Herein we present a novel, reliable method of furnishing antibody conjugates with a loading of two modules starting from a native antibody scaffold. Royal Society of Chemistry 2017-03-01 2016-11-28 /pmc/articles/PMC5399535/ /pubmed/28451324 http://dx.doi.org/10.1039/c6sc03655d Text en This journal is © The Royal Society of Chemistry 2016 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Lee, Maximillian T. W.
Maruani, Antoine
Richards, Daniel A.
Baker, James R.
Caddick, Stephen
Chudasama, Vijay
Enabling the controlled assembly of antibody conjugates with a loading of two modules without antibody engineering
title Enabling the controlled assembly of antibody conjugates with a loading of two modules without antibody engineering
title_full Enabling the controlled assembly of antibody conjugates with a loading of two modules without antibody engineering
title_fullStr Enabling the controlled assembly of antibody conjugates with a loading of two modules without antibody engineering
title_full_unstemmed Enabling the controlled assembly of antibody conjugates with a loading of two modules without antibody engineering
title_short Enabling the controlled assembly of antibody conjugates with a loading of two modules without antibody engineering
title_sort enabling the controlled assembly of antibody conjugates with a loading of two modules without antibody engineering
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5399535/
https://www.ncbi.nlm.nih.gov/pubmed/28451324
http://dx.doi.org/10.1039/c6sc03655d
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