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Adiponectin Deficiency Impairs Maternal Metabolic Adaptation to Pregnancy in Mice
Hypoadiponectinemia has been widely observed in patients with gestational diabetes mellitus (GDM). To investigate the causal role of hypoadiponectinemia in GDM, adiponectin gene knockout (Adipoq(−/−)) and wild-type (WT) mice were crossed to produce pregnant mouse models with or without adiponectin d...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Diabetes Association
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5399613/ https://www.ncbi.nlm.nih.gov/pubmed/28073830 http://dx.doi.org/10.2337/db16-1096 |
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author | Qiao, Liping Wattez, Jean-Sebastien Lee, Samuel Nguyen, Amanda Schaack, Jerome Hay, William W. Shao, Jianhua |
author_facet | Qiao, Liping Wattez, Jean-Sebastien Lee, Samuel Nguyen, Amanda Schaack, Jerome Hay, William W. Shao, Jianhua |
author_sort | Qiao, Liping |
collection | PubMed |
description | Hypoadiponectinemia has been widely observed in patients with gestational diabetes mellitus (GDM). To investigate the causal role of hypoadiponectinemia in GDM, adiponectin gene knockout (Adipoq(−/−)) and wild-type (WT) mice were crossed to produce pregnant mouse models with or without adiponectin deficiency. Adenoviral vector–mediated in vivo transduction was used to reconstitute adiponectin during late pregnancy. Results showed that Adipoq(−/−) dams developed glucose intolerance and hyperlipidemia in late pregnancy. Increased fetal body weight was detected in Adipoq(−/−) dams. Adiponectin reconstitution abolished these metabolic defects in Adipoq(−/−) dams. Hepatic glucose and triglyceride production rates of Adipoq(−/−) dams were significantly higher than those of WT dams. Robustly enhanced lipolysis was found in gonadal fat of Adipoq(−/−) dams. Interestingly, similar levels of insulin-induced glucose disposal and insulin signaling in metabolically active tissues in Adipoq(−/−) and WT dams indicated that maternal adiponectin deficiency does not reduce insulin sensitivity. However, remarkably decreased serum insulin concentrations were observed in Adipoq(−/−) dams. Furthermore, β-cell mass, but not glucose-stimulated insulin release, in Adipoq(−/−) dams was significantly reduced compared with WT dams. Together, these results demonstrate that adiponectin plays an important role in controlling maternal metabolic adaptation to pregnancy. |
format | Online Article Text |
id | pubmed-5399613 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | American Diabetes Association |
record_format | MEDLINE/PubMed |
spelling | pubmed-53996132018-05-01 Adiponectin Deficiency Impairs Maternal Metabolic Adaptation to Pregnancy in Mice Qiao, Liping Wattez, Jean-Sebastien Lee, Samuel Nguyen, Amanda Schaack, Jerome Hay, William W. Shao, Jianhua Diabetes Metabolism Hypoadiponectinemia has been widely observed in patients with gestational diabetes mellitus (GDM). To investigate the causal role of hypoadiponectinemia in GDM, adiponectin gene knockout (Adipoq(−/−)) and wild-type (WT) mice were crossed to produce pregnant mouse models with or without adiponectin deficiency. Adenoviral vector–mediated in vivo transduction was used to reconstitute adiponectin during late pregnancy. Results showed that Adipoq(−/−) dams developed glucose intolerance and hyperlipidemia in late pregnancy. Increased fetal body weight was detected in Adipoq(−/−) dams. Adiponectin reconstitution abolished these metabolic defects in Adipoq(−/−) dams. Hepatic glucose and triglyceride production rates of Adipoq(−/−) dams were significantly higher than those of WT dams. Robustly enhanced lipolysis was found in gonadal fat of Adipoq(−/−) dams. Interestingly, similar levels of insulin-induced glucose disposal and insulin signaling in metabolically active tissues in Adipoq(−/−) and WT dams indicated that maternal adiponectin deficiency does not reduce insulin sensitivity. However, remarkably decreased serum insulin concentrations were observed in Adipoq(−/−) dams. Furthermore, β-cell mass, but not glucose-stimulated insulin release, in Adipoq(−/−) dams was significantly reduced compared with WT dams. Together, these results demonstrate that adiponectin plays an important role in controlling maternal metabolic adaptation to pregnancy. American Diabetes Association 2017-05 2017-01-10 /pmc/articles/PMC5399613/ /pubmed/28073830 http://dx.doi.org/10.2337/db16-1096 Text en © 2017 by the American Diabetes Association. http://www.diabetesjournals.org/content/licenseReaders may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. More information is available at http://www.diabetesjournals.org/content/license. |
spellingShingle | Metabolism Qiao, Liping Wattez, Jean-Sebastien Lee, Samuel Nguyen, Amanda Schaack, Jerome Hay, William W. Shao, Jianhua Adiponectin Deficiency Impairs Maternal Metabolic Adaptation to Pregnancy in Mice |
title | Adiponectin Deficiency Impairs Maternal Metabolic Adaptation to Pregnancy in Mice |
title_full | Adiponectin Deficiency Impairs Maternal Metabolic Adaptation to Pregnancy in Mice |
title_fullStr | Adiponectin Deficiency Impairs Maternal Metabolic Adaptation to Pregnancy in Mice |
title_full_unstemmed | Adiponectin Deficiency Impairs Maternal Metabolic Adaptation to Pregnancy in Mice |
title_short | Adiponectin Deficiency Impairs Maternal Metabolic Adaptation to Pregnancy in Mice |
title_sort | adiponectin deficiency impairs maternal metabolic adaptation to pregnancy in mice |
topic | Metabolism |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5399613/ https://www.ncbi.nlm.nih.gov/pubmed/28073830 http://dx.doi.org/10.2337/db16-1096 |
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