MiR-519d impedes cisplatin-resistance in breast cancer stem cells by down-regulating the expression of MCL-1

Cancer stem cells are considered as the cell population which is responsible for chemoresistance and treatment failure in breast cancer patients. Therefore, it is urgent to explore the mechanism by which cancer stem cells survive under the treatment of chemotherapeutic drugs such as cisplatin. In th...

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Autores principales: Xie, Qing, Wang, Shuai, Zhao, Yue, Zhang, Zhenchao, Qin, Chuan, Yang, Xianjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5400641/
https://www.ncbi.nlm.nih.gov/pubmed/28423543
http://dx.doi.org/10.18632/oncotarget.15781
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author Xie, Qing
Wang, Shuai
Zhao, Yue
Zhang, Zhenchao
Qin, Chuan
Yang, Xianjun
author_facet Xie, Qing
Wang, Shuai
Zhao, Yue
Zhang, Zhenchao
Qin, Chuan
Yang, Xianjun
author_sort Xie, Qing
collection PubMed
description Cancer stem cells are considered as the cell population which is responsible for chemoresistance and treatment failure in breast cancer patients. Therefore, it is urgent to explore the mechanism by which cancer stem cells survive under the treatment of chemotherapeutic drugs such as cisplatin. In this paper, we demonstrated significant decrease of miR-519d in breast cancer stem cells by quantitative RT-PCR analysis. Furthermore, we found the enforced expression of miR-519d in T-47D-cancer stem cells significantly increased their sensitivity to cisplatin through the apoptosis pathway. In addition, the gene of MCL-1, which is a member of pro-apoptotic Bcl-2 family, was found to be the target of miR-519d in T-47D-cancer stem cells. Our date demonstrated that enforced miR-519d expression enhanced the cisplatin-induced apoptosis through the MCL-1 dependent mitochondria pathway in breast cancer stem cells. Taken together, the present study suggests that miR-519d reduces chemoresistance in breast cancer stem cells, and understanding of miR-519d may be helpful for increasing the efficacy of chemotherapy.
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spelling pubmed-54006412017-05-03 MiR-519d impedes cisplatin-resistance in breast cancer stem cells by down-regulating the expression of MCL-1 Xie, Qing Wang, Shuai Zhao, Yue Zhang, Zhenchao Qin, Chuan Yang, Xianjun Oncotarget Research Paper Cancer stem cells are considered as the cell population which is responsible for chemoresistance and treatment failure in breast cancer patients. Therefore, it is urgent to explore the mechanism by which cancer stem cells survive under the treatment of chemotherapeutic drugs such as cisplatin. In this paper, we demonstrated significant decrease of miR-519d in breast cancer stem cells by quantitative RT-PCR analysis. Furthermore, we found the enforced expression of miR-519d in T-47D-cancer stem cells significantly increased their sensitivity to cisplatin through the apoptosis pathway. In addition, the gene of MCL-1, which is a member of pro-apoptotic Bcl-2 family, was found to be the target of miR-519d in T-47D-cancer stem cells. Our date demonstrated that enforced miR-519d expression enhanced the cisplatin-induced apoptosis through the MCL-1 dependent mitochondria pathway in breast cancer stem cells. Taken together, the present study suggests that miR-519d reduces chemoresistance in breast cancer stem cells, and understanding of miR-519d may be helpful for increasing the efficacy of chemotherapy. Impact Journals LLC 2017-02-28 /pmc/articles/PMC5400641/ /pubmed/28423543 http://dx.doi.org/10.18632/oncotarget.15781 Text en Copyright: © 2017 Xie et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Research Paper
Xie, Qing
Wang, Shuai
Zhao, Yue
Zhang, Zhenchao
Qin, Chuan
Yang, Xianjun
MiR-519d impedes cisplatin-resistance in breast cancer stem cells by down-regulating the expression of MCL-1
title MiR-519d impedes cisplatin-resistance in breast cancer stem cells by down-regulating the expression of MCL-1
title_full MiR-519d impedes cisplatin-resistance in breast cancer stem cells by down-regulating the expression of MCL-1
title_fullStr MiR-519d impedes cisplatin-resistance in breast cancer stem cells by down-regulating the expression of MCL-1
title_full_unstemmed MiR-519d impedes cisplatin-resistance in breast cancer stem cells by down-regulating the expression of MCL-1
title_short MiR-519d impedes cisplatin-resistance in breast cancer stem cells by down-regulating the expression of MCL-1
title_sort mir-519d impedes cisplatin-resistance in breast cancer stem cells by down-regulating the expression of mcl-1
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5400641/
https://www.ncbi.nlm.nih.gov/pubmed/28423543
http://dx.doi.org/10.18632/oncotarget.15781
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