Vitrification after multiple rounds of sample application and blotting improves particle density on cryo-electron microscopy grids

Single particle cryo-electron microscopy (cryoEM) is becoming widely adopted as a tool for structural characterization of biomolecules at near-atomic resolution. Vitrification of the sample to obtain a dense distribution of particles within a single field of view remains a major bottleneck for the s...

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Detalles Bibliográficos
Autores principales: Snijder, Joost, Borst, Andrew J., Dosey, Annie, Walls, Alexandra C., Burrell, Anika, Reddy, Vijay S., Kollman, Justin M., Veesler, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5400742/
https://www.ncbi.nlm.nih.gov/pubmed/28254381
http://dx.doi.org/10.1016/j.jsb.2017.02.008

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5400742/
https://www.ncbi.nlm.nih.gov/pubmed/28254381
http://dx.doi.org/10.1016/j.jsb.2017.02.008

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