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Vitrification after multiple rounds of sample application and blotting improves particle density on cryo-electron microscopy grids
Single particle cryo-electron microscopy (cryoEM) is becoming widely adopted as a tool for structural characterization of biomolecules at near-atomic resolution. Vitrification of the sample to obtain a dense distribution of particles within a single field of view remains a major bottleneck for the s...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier Inc.
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5400742/ https://www.ncbi.nlm.nih.gov/pubmed/28254381 http://dx.doi.org/10.1016/j.jsb.2017.02.008 |