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Optimizing Immunostaining of Enamel Matrix: Application of Sudan Black B and Minimization of False Positives from Normal Sera and IgGs

Non-specific fluorescence from demineralized enamel matrix can significantly compromise the immunofluorescence studies and lead to false positives. Our goal was to assess degrees of non-specific binding under different conditions and try to optimize procedures for immunofluorescence studies of formi...

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Detalles Bibliográficos
Autores principales: Yang, Xu, Vidunas, Alexander J., Beniash, Elia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5403949/
https://www.ncbi.nlm.nih.gov/pubmed/28487659
http://dx.doi.org/10.3389/fphys.2017.00239