Heritable Genomic Fragment Deletions and Small Indels in the Putative ENGase Gene Induced by CRISPR/Cas9 in Barley

Targeted genome editing with the CRISPR/Cas9 system has been used extensively for the selective mutation of plant genes. Here we used CRISPR/Cas9 to disrupt the putative barley (Hordeum vulgare cv. “Golden Promise”) endo-N-acetyl-β-D-glucosaminidase (ENGase) gene. Five single guide RNAs (sgRNAs) wer...

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Detalles Bibliográficos
Autores principales: Kapusi, Eszter, Corcuera-Gómez, Maria, Melnik, Stanislav, Stoger, Eva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5404177/
https://www.ncbi.nlm.nih.gov/pubmed/28487703
http://dx.doi.org/10.3389/fpls.2017.00540

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5404177/
https://www.ncbi.nlm.nih.gov/pubmed/28487703
http://dx.doi.org/10.3389/fpls.2017.00540

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