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Proportions of circulating follicular helper T cells are reduced and correlate with memory B cells in HIV-infected children
INTRODUCTION: HIV causes defects in memory B cells in children, but the mechanisms of those defects have not been fully elucidated. One possible mechanism is the lack of T-cell help to B cells during immune reactions. However, few studies have assessed the effect of HIV on follicular helper T cells...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5405965/ https://www.ncbi.nlm.nih.gov/pubmed/28445512 http://dx.doi.org/10.1371/journal.pone.0175570 |
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author | Muema, Daniel M. Macharia, Gladys N. Olusola, Babatunde A. Hassan, Amin S. Fegan, Greg W. Berkley, James A. Urban, Britta C. Nduati, Eunice W. |
author_facet | Muema, Daniel M. Macharia, Gladys N. Olusola, Babatunde A. Hassan, Amin S. Fegan, Greg W. Berkley, James A. Urban, Britta C. Nduati, Eunice W. |
author_sort | Muema, Daniel M. |
collection | PubMed |
description | INTRODUCTION: HIV causes defects in memory B cells in children, but the mechanisms of those defects have not been fully elucidated. One possible mechanism is the lack of T-cell help to B cells during immune reactions. However, few studies have assessed the effect of HIV on follicular helper T cells (T(FH) cells) in children. METHODS: In this study, follicular-homing CD4 T cells and memory B cells were assessed in HIV-infected children and compared with children from the community. CXCR5 and CD45RO were used as markers of follicular-homing T cells and memory T cells, respectively. Memory T(FH) cells were identified as CD3(+)CD8(-)CD4(+)CXCR5(+)CD45RO(+)PD1(+). Central memory T cells were identified based on CCR7 expression. Relationship between the proportions of follicular-homing CD4 T cells and memory B cells were determined in multivariable regression models. RESULTS: Highly viremic HIV-infected children had lower proportions of memory T(FH) cells when compared with community control children. In multivariable analyses, high proportions of memory T(FH) cells were associated with increased percentages of resting memory B cells after adjusting for other covariates. CONCLUSION: The impact of HIV on follicular helper T cells could influence the accumulation of memory B cells in HIV-infected children. |
format | Online Article Text |
id | pubmed-5405965 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-54059652017-05-14 Proportions of circulating follicular helper T cells are reduced and correlate with memory B cells in HIV-infected children Muema, Daniel M. Macharia, Gladys N. Olusola, Babatunde A. Hassan, Amin S. Fegan, Greg W. Berkley, James A. Urban, Britta C. Nduati, Eunice W. PLoS One Research Article INTRODUCTION: HIV causes defects in memory B cells in children, but the mechanisms of those defects have not been fully elucidated. One possible mechanism is the lack of T-cell help to B cells during immune reactions. However, few studies have assessed the effect of HIV on follicular helper T cells (T(FH) cells) in children. METHODS: In this study, follicular-homing CD4 T cells and memory B cells were assessed in HIV-infected children and compared with children from the community. CXCR5 and CD45RO were used as markers of follicular-homing T cells and memory T cells, respectively. Memory T(FH) cells were identified as CD3(+)CD8(-)CD4(+)CXCR5(+)CD45RO(+)PD1(+). Central memory T cells were identified based on CCR7 expression. Relationship between the proportions of follicular-homing CD4 T cells and memory B cells were determined in multivariable regression models. RESULTS: Highly viremic HIV-infected children had lower proportions of memory T(FH) cells when compared with community control children. In multivariable analyses, high proportions of memory T(FH) cells were associated with increased percentages of resting memory B cells after adjusting for other covariates. CONCLUSION: The impact of HIV on follicular helper T cells could influence the accumulation of memory B cells in HIV-infected children. Public Library of Science 2017-04-26 /pmc/articles/PMC5405965/ /pubmed/28445512 http://dx.doi.org/10.1371/journal.pone.0175570 Text en © 2017 Muema et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Muema, Daniel M. Macharia, Gladys N. Olusola, Babatunde A. Hassan, Amin S. Fegan, Greg W. Berkley, James A. Urban, Britta C. Nduati, Eunice W. Proportions of circulating follicular helper T cells are reduced and correlate with memory B cells in HIV-infected children |
title | Proportions of circulating follicular helper T cells are reduced and correlate with memory B cells in HIV-infected children |
title_full | Proportions of circulating follicular helper T cells are reduced and correlate with memory B cells in HIV-infected children |
title_fullStr | Proportions of circulating follicular helper T cells are reduced and correlate with memory B cells in HIV-infected children |
title_full_unstemmed | Proportions of circulating follicular helper T cells are reduced and correlate with memory B cells in HIV-infected children |
title_short | Proportions of circulating follicular helper T cells are reduced and correlate with memory B cells in HIV-infected children |
title_sort | proportions of circulating follicular helper t cells are reduced and correlate with memory b cells in hiv-infected children |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5405965/ https://www.ncbi.nlm.nih.gov/pubmed/28445512 http://dx.doi.org/10.1371/journal.pone.0175570 |
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