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Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System

Single-molecule Förster Resonance Energy Transfer (smFRET) can be used to obtain structural information on biomolecular complexes in real-time. Thereby, multiple smFRET measurements are used to localize an unknown dye position inside a protein complex by means of trilateration. In order to obtain qu...

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Detalles Bibliográficos
Autores principales: Dörfler, Thilo, Eilert, Tobias, Röcker, Carlheinz, Nagy, Julia, Michaelis, Jens
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5407667/
https://www.ncbi.nlm.nih.gov/pubmed/28287526
http://dx.doi.org/10.3791/54782