STUDIES ON THE INTERACTION BETWEEN TRIPTOLIDE AND BOVINE SERUM ALBUMIN (BSA) BY SPECTROSCOPIC AND MOLECULAR MODELING METHODS

BACKGROUND: Triptolide is a major active constituent isolated from Tripterygiumwilfordii Hook F, a Chinese herbal medicine. This study investigated the intermolecular interaction between triptolide and bovine serum albumin (BSA). MATERIALS AND METHODS: The fluorescence, circular dichroism (CD) and m...

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Autores principales: Wang, Haidong, Shi, Hailang, Pang, Jie, Song, Xingfa, Xu, Caiyun, Sun, Zengxian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: African Traditional Herbal Medicine Supporters Initiative (ATHMSI) 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412182/
https://www.ncbi.nlm.nih.gov/pubmed/28480368
http://dx.doi.org/10.21010/ajtcam.v13i6.17
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author Wang, Haidong
Shi, Hailang
Pang, Jie
Song, Xingfa
Xu, Caiyun
Sun, Zengxian
author_facet Wang, Haidong
Shi, Hailang
Pang, Jie
Song, Xingfa
Xu, Caiyun
Sun, Zengxian
author_sort Wang, Haidong
collection PubMed
description BACKGROUND: Triptolide is a major active constituent isolated from Tripterygiumwilfordii Hook F, a Chinese herbal medicine. This study investigated the intermolecular interaction between triptolide and bovine serum albumin (BSA). MATERIALS AND METHODS: The fluorescence, circular dichroism (CD) and molecular docking methods were used to investigate the intermolecular interaction between triptolide and BSA. The binding constant, the number of binding sites, binding subdomain and the thermodynamic parameters were measured. RESULTS: The results of this experiment revealed that the intrinsic fluorescence of BSA was effectively quenched by triptolide via static quenching. The experimental results of synchronous fluorescence and CD spectra showed that the conformation of BSA was changed in the presence of triptolide. CONCLUSION: It indicated that triptolide could spontaneously bind on site II (subdomain IIIA) of BSA mainly via hydrogen bonding interactions and Van der Waals force.
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spelling pubmed-54121822017-09-29 STUDIES ON THE INTERACTION BETWEEN TRIPTOLIDE AND BOVINE SERUM ALBUMIN (BSA) BY SPECTROSCOPIC AND MOLECULAR MODELING METHODS Wang, Haidong Shi, Hailang Pang, Jie Song, Xingfa Xu, Caiyun Sun, Zengxian Afr J Tradit Complement Altern Med Article BACKGROUND: Triptolide is a major active constituent isolated from Tripterygiumwilfordii Hook F, a Chinese herbal medicine. This study investigated the intermolecular interaction between triptolide and bovine serum albumin (BSA). MATERIALS AND METHODS: The fluorescence, circular dichroism (CD) and molecular docking methods were used to investigate the intermolecular interaction between triptolide and BSA. The binding constant, the number of binding sites, binding subdomain and the thermodynamic parameters were measured. RESULTS: The results of this experiment revealed that the intrinsic fluorescence of BSA was effectively quenched by triptolide via static quenching. The experimental results of synchronous fluorescence and CD spectra showed that the conformation of BSA was changed in the presence of triptolide. CONCLUSION: It indicated that triptolide could spontaneously bind on site II (subdomain IIIA) of BSA mainly via hydrogen bonding interactions and Van der Waals force. African Traditional Herbal Medicine Supporters Initiative (ATHMSI) 2016-09-29 /pmc/articles/PMC5412182/ /pubmed/28480368 http://dx.doi.org/10.21010/ajtcam.v13i6.17 Text en Copyright: © 2016 Afr. J. Traditional Complementary and Alternative Medicines http://creativecommons.org/licenses/CC-BY/4.0 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
spellingShingle Article
Wang, Haidong
Shi, Hailang
Pang, Jie
Song, Xingfa
Xu, Caiyun
Sun, Zengxian
STUDIES ON THE INTERACTION BETWEEN TRIPTOLIDE AND BOVINE SERUM ALBUMIN (BSA) BY SPECTROSCOPIC AND MOLECULAR MODELING METHODS
title STUDIES ON THE INTERACTION BETWEEN TRIPTOLIDE AND BOVINE SERUM ALBUMIN (BSA) BY SPECTROSCOPIC AND MOLECULAR MODELING METHODS
title_full STUDIES ON THE INTERACTION BETWEEN TRIPTOLIDE AND BOVINE SERUM ALBUMIN (BSA) BY SPECTROSCOPIC AND MOLECULAR MODELING METHODS
title_fullStr STUDIES ON THE INTERACTION BETWEEN TRIPTOLIDE AND BOVINE SERUM ALBUMIN (BSA) BY SPECTROSCOPIC AND MOLECULAR MODELING METHODS
title_full_unstemmed STUDIES ON THE INTERACTION BETWEEN TRIPTOLIDE AND BOVINE SERUM ALBUMIN (BSA) BY SPECTROSCOPIC AND MOLECULAR MODELING METHODS
title_short STUDIES ON THE INTERACTION BETWEEN TRIPTOLIDE AND BOVINE SERUM ALBUMIN (BSA) BY SPECTROSCOPIC AND MOLECULAR MODELING METHODS
title_sort studies on the interaction between triptolide and bovine serum albumin (bsa) by spectroscopic and molecular modeling methods
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412182/
https://www.ncbi.nlm.nih.gov/pubmed/28480368
http://dx.doi.org/10.21010/ajtcam.v13i6.17
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