Construction and optimization of an efficient amplification method of a random ssDNA library by asymmetric emulsion PCR

Construction of a random ssDNA sublibrary is an important step of the aptamer screening process. The available construction methods include asymmetric PCR, biotin–streptavidin separation, and lambda exonuclease digestions, in which PCR amplification is a key step. The main drawback of PCR amplificat...

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Detalles Bibliográficos
Autores principales: Shao, Keke, Shi, Xinhui, Zhu, Xiangjun, Cui, Leilei, Shao, Qixiang, Ma, Da
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Original Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412896/
https://www.ncbi.nlm.nih.gov/pubmed/26671010
http://dx.doi.org/10.1002/bab.1467

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412896/
https://www.ncbi.nlm.nih.gov/pubmed/26671010
http://dx.doi.org/10.1002/bab.1467

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