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Construction and optimization of an efficient amplification method of a random ssDNA library by asymmetric emulsion PCR
Construction of a random ssDNA sublibrary is an important step of the aptamer screening process. The available construction methods include asymmetric PCR, biotin–streptavidin separation, and lambda exonuclease digestions, in which PCR amplification is a key step. The main drawback of PCR amplificat...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412896/ https://www.ncbi.nlm.nih.gov/pubmed/26671010 http://dx.doi.org/10.1002/bab.1467 |