Cargando…

Enzyme discovery beyond homology: a unique hydroxynitrile lyase in the Bet v1 superfamily

Homology and similarity based approaches are most widely used for the identification of new enzymes for biocatalysis. However, they are not suitable to find truly novel scaffolds with a desired function and this averts options and diversity. Hydroxynitrile lyases (HNLs) are an example of non-homolog...

Descripción completa

Detalles Bibliográficos
Autores principales:	Lanfranchi, Elisa, Pavkov-Keller, Tea, Koehler, Eva-Maria, Diepold, Matthias, Steiner, Kerstin, Darnhofer, Barbara, Hartler, Jürgen, Van Den Bergh, Tom, Joosten, Henk-Jan, Gruber-Khadjawi, Mandana, Thallinger, Gerhard G., Birner-Gruenberger, Ruth, Gruber, Karl, Winkler, Margit, Glieder, Anton
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5413884/ https://www.ncbi.nlm.nih.gov/pubmed/28466867 http://dx.doi.org/10.1038/srep46738

Ejemplares similares

Erratum: Enzyme discovery beyond homology: a unique hydroxynitrile lyase in the Bet v1 superfamily
por: Lanfranchi, Elisa, et al.
Publicado: (2017)

Discovery of a novel (R)-selective bacterial hydroxynitrile lyase from Acidobacterium capsulatum
por: Wiedner, Romana, et al.
Publicado: (2014)

Substrate Binding in the FAD-Dependent Hydroxynitrile Lyase from Almond Provides Insight into the Mechanism of Cyanohydrin Formation and Explains the Absence of Dehydrogenation Activity(,)
por: Dreveny, Ingrid, et al.
Publicado: (2009)

Crystal Structure and Catalytic Mechanism of CouO, a Versatile C-Methyltransferase from Streptomyces rishiriensis
por: Pavkov-Keller, Tea, et al.
Publicado: (2017)

Activity and Enantioselectivity of the Hydroxynitrile Lyase MeHNL in Dry Organic Solvents
por: Paravidino, Monica, et al.
Publicado: (2010)

Progress in Stereoselective Construction of C–C Bonds Enabled by Aldolases and Hydroxynitrile Lyases
por: Liu, Mi, et al.
Publicado: (2021)

Hydroxynitrile Lyases with α/β-Hydrolase Fold: Two Enzymes with Almost Identical 3D Structures but Opposite Enantioselectivities and Different Reaction Mechanisms
por: Andexer, Jennifer N, et al.
Publicado: (2012)

Protein evolution analysis of S-hydroxynitrile lyase by complete sequence design utilizing the INTMSAlign software
por: Nakano, Shogo, et al.
Publicado: (2015)

Larger active site in an ancestral hydroxynitrile lyase increases catalytically promiscuous esterase activity
por: Jones, Bryan J., et al.
Publicado: (2020)

Designing Efficient Enzymes: Eight Predicted Mutations Convert a Hydroxynitrile Lyase into an Efficient Esterase
por: Casadevall, Guillem, et al.
Publicado: (2023)

The 2.5 Å Structure of the Enterococcus Conjugation Protein TraM resembles VirB8 Type IV Secretion Proteins
por: Goessweiner-Mohr, Nikolaus, et al.
Publicado: (2013)

Overexpression of Hydroxynitrile Lyase in Cassava Roots Elevates Protein and Free Amino Acids while Reducing Residual Cyanogen Levels
por: Narayanan, Narayanan N., et al.
Publicado: (2011)

Hydroxynitrile lyases from cyanogenic millipedes: molecular cloning, heterologous expression, and whole-cell biocatalysis for the production of (R)-mandelonitrile
por: Yamaguchi, Takuya, et al.
Publicado: (2018)

Discovery and Structural Analysis to Improve the Enantioselectivity of Hydroxynitrile Lyase from Parafontaria laminata Millipedes for (R)-2-Chloromandelonitrile Synthesis
por: Nuylert, Aem, et al.
Publicado: (2020)

R‐hydroxynitrile lyase from the cyanogenic millipede, Chamberlinius hualienensis—A new entry to the carrier protein family Lipocalines
por: Motojima, Fumihiro, et al.
Publicado: (2020)

Nasal mucus proteome and its involvement in allergic rhinitis
por: Tomazic, Peter Valentin, et al.
Publicado: (2020)

Structural characterization of Linum usitatissimum hydroxynitrile lyase: A new cyanohydrin decomposition mechanism involving a cyano-zinc complex
por: Zheng, Daijun, et al.
Publicado: (2022)

The cyanogenic syndrome in rubber tree Hevea brasiliensis: tissue-damage-dependent activation of linamarase and hydroxynitrile lyase accelerates hydrogen cyanide release
por: Kadow, Daniel, et al.
Publicado: (2012)

Crystallization and preliminary structure determination of the transfer protein TraM from the Gram-positive conjugative plasmid pIP501
por: Goessweiner-Mohr, Nikolaus, et al.
Publicado: (2013)

The Crystal Structure of Mouse Ces2c, a Potential Ortholog of Human CES2, Shows Structural Similarities in Substrate Regulation and Product Release to Human CES1
por: Eisner, Helgit, et al.
Publicado: (2022)

Identification of Key Residues for Enzymatic Carboxylate Reduction
por: Stolterfoht, Holly, et al.
Publicado: (2018)

Descriptive proteomics of paired human vocal fold and buccal mucosa tissue
por: Grossmann, Tanja, et al.
Publicado: (2021)

Discovery and structural characterisation of new fold type IV-transaminases exemplify the diversity of this enzyme fold
por: Pavkov-Keller, Tea, et al.
Publicado: (2016)

Overexpression of recombinant proteins containing non-canonical amino acids in Vibrio natriegens: p-azido-L-phenylalanine as coupling site for (19)F-tags
por: Stadler, Karina A., et al.
Publicado: (2022)

Comparative proteomics of paired vocal fold and oral mucosa fibroblasts
por: Karbiener, Michael, et al.
Publicado: (2017)

Comparative proteomics of common allergenic tree pollens of birch, alder, and hazel
por: Darnhofer, Barbara, et al.
Publicado: (2021)

Spatially Resolved Activity-based Proteomic Profiles of the Murine Small Intestinal Lipases
por: Schittmayer, Matthias, et al.
Publicado: (2020)

Hepatocyte Proteome Alterations Induced by Individual and Combinations of Common Free Fatty Acids
por: Gindlhuber, Juergen, et al.
Publicado: (2022)

Structure and biochemical properties of recombinant human dimethylglycine dehydrogenase and comparison to the disease‐related H109R variant
por: Augustin, Peter, et al.
Publicado: (2016)

Substituting the catalytic proline of 4-oxalocrotonate tautomerase with non-canonical analogues reveals a finely tuned catalytic system
por: Lukesch, Michael S., et al.
Publicado: (2019)

Rational Engineered C-Acyltransferase Transforms Sterically Demanding Acyl Donors
por: Żądło-Dobrowolska, Anna, et al.
Publicado: (2019)

Effect of Noncanonical Amino Acids on Protein–Carbohydrate Interactions: Structure, Dynamics, and Carbohydrate Affinity of a Lectin Engineered with Fluorinated Tryptophan Analogs
por: Tobola, Felix, et al.
Publicado: (2018)

Folding Assessment of Incorporation of Noncanonical Amino Acids Facilitates Expansion of Functional‐Group Diversity for Enzyme Engineering
por: Drienovská, Ivana, et al.
Publicado: (2020)

Introducing a new type of alternative laryngeal mucosa model
por: Grossmann, Tanja, et al.
Publicado: (2023)

Structure and Catalytic Mechanism of a Bacterial Friedel–Crafts Acylase
por: Pavkov‐Keller, Tea, et al.
Publicado: (2018)

Polyphenol oxidases exhibit promiscuous proteolytic activity
por: Biundo, A., et al.
Publicado: (2020)

Biocatalytic Friedel–Crafts Acylation and Fries Reaction
por: Schmidt, Nina G., et al.
Publicado: (2017)

Substrate complexes of human dipeptidyl peptidase III reveal the mechanism of enzyme inhibition
por: Kumar, Prashant, et al.
Publicado: (2016)

Diversified glucosinolate metabolism: biosynthesis of hydrogen cyanide and of the hydroxynitrile glucoside alliarinoside in relation to sinigrin metabolism in Alliaria petiolata
por: Frisch, Tina, et al.
Publicado: (2015)

Conformational Plasticity and Ligand Binding of Bacterial Monoacylglycerol Lipase
por: Rengachari, Srinivasan, et al.
Publicado: (2013)

Cannot write session to /tmp/vufind_sessions/sess_jh419ktdokvikhvj91ch5faa9g