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Quantitative longitudinal imaging of activated microglia as a marker of inflammation in the pilocarpine rat model of epilepsy using [(11)C]-(R)-PK11195 PET and MRI
Inflammation may play a role in the development of epilepsy after brain insults. [(11)C]-(R)-PK11195 binds to TSPO, expressed by activated microglia. We quantified [(11)C]-(R)-PK11195 binding during epileptogenesis after pilocarpine-induced status epilepticus (SE), a model of temporal lobe epilepsy....
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5414902/ https://www.ncbi.nlm.nih.gov/pubmed/27381824 http://dx.doi.org/10.1177/0271678X16653615 |
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author | Yankam Njiwa, J Costes, N Bouillot, C Bouvard, S Fieux, S Becker, G Levigoureux, E Kocevar, G Stamile, C Langlois, JB Bolbos, R Bonnet, C Bezin, L Zimmer, L Hammers, A |
author_facet | Yankam Njiwa, J Costes, N Bouillot, C Bouvard, S Fieux, S Becker, G Levigoureux, E Kocevar, G Stamile, C Langlois, JB Bolbos, R Bonnet, C Bezin, L Zimmer, L Hammers, A |
author_sort | Yankam Njiwa, J |
collection | PubMed |
description | Inflammation may play a role in the development of epilepsy after brain insults. [(11)C]-(R)-PK11195 binds to TSPO, expressed by activated microglia. We quantified [(11)C]-(R)-PK11195 binding during epileptogenesis after pilocarpine-induced status epilepticus (SE), a model of temporal lobe epilepsy. Nine male rats were studied thrice (D0-1, D0 + 6, D0 + 35, D0 = SE induction). In the same session, 7T T2-weighted images and DTI for mean diffusivity (MD) and fractional anisotropy (FA) maps were acquired, followed by dynamic PET/CT. On D0 + 35, femoral arterial blood was sampled for rat-specific metabolite-corrected arterial plasma input functions (AIFs). In multiple MR-derived ROIs, we assessed four kinetic models (two with AIFs; two using a reference region), standard uptake values (SUVs), and a model with a mean AIF. All models showed large (up to two-fold) and significant TSPO binding increases in regions expected to be affected, and comparatively little change in the brainstem, at D0 + 6. Some individuals showed increases at D0 + 35. AIF models yielded more consistent increases at D0 + 6. FA values were decreased at D0 + 6 and had recovered by D0 + 35. MD was increased at D0 + 6 and more so at D0 + 35. [(11)C]-(R)-PK11195 PET binding and MR biomarker changes could be detected with only nine rats, highlighting the potential of longitudinal imaging studies. |
format | Online Article Text |
id | pubmed-5414902 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-54149022017-05-11 Quantitative longitudinal imaging of activated microglia as a marker of inflammation in the pilocarpine rat model of epilepsy using [(11)C]-(R)-PK11195 PET and MRI Yankam Njiwa, J Costes, N Bouillot, C Bouvard, S Fieux, S Becker, G Levigoureux, E Kocevar, G Stamile, C Langlois, JB Bolbos, R Bonnet, C Bezin, L Zimmer, L Hammers, A J Cereb Blood Flow Metab Original Articles Inflammation may play a role in the development of epilepsy after brain insults. [(11)C]-(R)-PK11195 binds to TSPO, expressed by activated microglia. We quantified [(11)C]-(R)-PK11195 binding during epileptogenesis after pilocarpine-induced status epilepticus (SE), a model of temporal lobe epilepsy. Nine male rats were studied thrice (D0-1, D0 + 6, D0 + 35, D0 = SE induction). In the same session, 7T T2-weighted images and DTI for mean diffusivity (MD) and fractional anisotropy (FA) maps were acquired, followed by dynamic PET/CT. On D0 + 35, femoral arterial blood was sampled for rat-specific metabolite-corrected arterial plasma input functions (AIFs). In multiple MR-derived ROIs, we assessed four kinetic models (two with AIFs; two using a reference region), standard uptake values (SUVs), and a model with a mean AIF. All models showed large (up to two-fold) and significant TSPO binding increases in regions expected to be affected, and comparatively little change in the brainstem, at D0 + 6. Some individuals showed increases at D0 + 35. AIF models yielded more consistent increases at D0 + 6. FA values were decreased at D0 + 6 and had recovered by D0 + 35. MD was increased at D0 + 6 and more so at D0 + 35. [(11)C]-(R)-PK11195 PET binding and MR biomarker changes could be detected with only nine rats, highlighting the potential of longitudinal imaging studies. SAGE Publications 2016-01-01 2017-04 /pmc/articles/PMC5414902/ /pubmed/27381824 http://dx.doi.org/10.1177/0271678X16653615 Text en © The Author(s) 2016 http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution 3.0 License (http://www.creativecommons.org/licenses/by/3.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Articles Yankam Njiwa, J Costes, N Bouillot, C Bouvard, S Fieux, S Becker, G Levigoureux, E Kocevar, G Stamile, C Langlois, JB Bolbos, R Bonnet, C Bezin, L Zimmer, L Hammers, A Quantitative longitudinal imaging of activated microglia as a marker of inflammation in the pilocarpine rat model of epilepsy using [(11)C]-(R)-PK11195 PET and MRI |
title | Quantitative longitudinal imaging of activated microglia as a marker of inflammation in the pilocarpine rat model of epilepsy using [(11)C]-(R)-PK11195 PET and MRI |
title_full | Quantitative longitudinal imaging of activated microglia as a marker of inflammation in the pilocarpine rat model of epilepsy using [(11)C]-(R)-PK11195 PET and MRI |
title_fullStr | Quantitative longitudinal imaging of activated microglia as a marker of inflammation in the pilocarpine rat model of epilepsy using [(11)C]-(R)-PK11195 PET and MRI |
title_full_unstemmed | Quantitative longitudinal imaging of activated microglia as a marker of inflammation in the pilocarpine rat model of epilepsy using [(11)C]-(R)-PK11195 PET and MRI |
title_short | Quantitative longitudinal imaging of activated microglia as a marker of inflammation in the pilocarpine rat model of epilepsy using [(11)C]-(R)-PK11195 PET and MRI |
title_sort | quantitative longitudinal imaging of activated microglia as a marker of inflammation in the pilocarpine rat model of epilepsy using [(11)c]-(r)-pk11195 pet and mri |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5414902/ https://www.ncbi.nlm.nih.gov/pubmed/27381824 http://dx.doi.org/10.1177/0271678X16653615 |
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