A One-Step PCR-Based Assay to Evaluate the Efficiency and Precision of Genomic DNA-Editing Tools

Despite rapid progress, many problems and limitations persist and limit the applicability of gene-editing techniques. Making use of meganucleases, TALENs, or CRISPR/Cas9-based tools requires an initial step of pre-screening to determine the efficiency and specificity of the designed tools. This step...

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Detalles Bibliográficos
Autores principales: Germini, Diego, Bou Saada, Yara, Tsfasman, Tatiana, Osina, Kristina, Robin, Chloé, Lomov, Nikolay, Rubtsov, Mikhail, Sjakste, Nikolajs, Lipinski, Mar≿, Vassetzky, Yegor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2017
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5415314/
https://www.ncbi.nlm.nih.gov/pubmed/28480303
http://dx.doi.org/10.1016/j.omtm.2017.03.001

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5415314/
https://www.ncbi.nlm.nih.gov/pubmed/28480303
http://dx.doi.org/10.1016/j.omtm.2017.03.001

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