CRISPR/Cas9-Directed Reassignment of the GATA1 Initiation Codon in K562 Cells to Recapitulate AML in Down Syndrome

Using a CRISPR/Cas9 system, we have reengineered a translational start site in the GATA1 gene in K562 cells. This mutation accounts largely for the onset of myeloid leukemia in Down syndrome (ML-DS). For this reengineering, we utilized CRISPR/Cas9 to generate mammalian cell lines that express trunca...

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Detalles Bibliográficos
Autores principales: Bloh, Kevin M., Bialk, Pawel A., Gopalakrishnapillai, Anilkumar, Kolb, E. Anders, Kmiec, Eric B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2017
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5415552/
https://www.ncbi.nlm.nih.gov/pubmed/28624204
http://dx.doi.org/10.1016/j.omtn.2017.04.009

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5415552/
https://www.ncbi.nlm.nih.gov/pubmed/28624204
http://dx.doi.org/10.1016/j.omtn.2017.04.009

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