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Development of a simplified RT-PCR without RNA isolation for rapid detection of RNA viruses in a single small brown planthopper (Laodelphax striatellus Fallén)

BACKGROUND: The small brown planthopper (SBPH) is an important pest of cereal crops and acts as a transmission vector for multiple RNA viruses. Rapid diagnosis of virus in the vector is crucial for efficient forecast and control of viral disease. Reverse transcription polymerase chain reaction (RT-P...

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Detalles Bibliográficos
Autores principales: Xu, Qiufang, Liu, Haoqiu, Yuan, Pingping, Zhang, Xiaoxia, Chen, Qingqing, Jiang, Xuanli, Zhou, Yijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5415734/
https://www.ncbi.nlm.nih.gov/pubmed/28468626
http://dx.doi.org/10.1186/s12985-017-0732-6