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Development of a simplified RT-PCR without RNA isolation for rapid detection of RNA viruses in a single small brown planthopper (Laodelphax striatellus Fallén)
BACKGROUND: The small brown planthopper (SBPH) is an important pest of cereal crops and acts as a transmission vector for multiple RNA viruses. Rapid diagnosis of virus in the vector is crucial for efficient forecast and control of viral disease. Reverse transcription polymerase chain reaction (RT-P...
Autores principales: | Xu, Qiufang, Liu, Haoqiu, Yuan, Pingping, Zhang, Xiaoxia, Chen, Qingqing, Jiang, Xuanli, Zhou, Yijun |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5415734/ https://www.ncbi.nlm.nih.gov/pubmed/28468626 http://dx.doi.org/10.1186/s12985-017-0732-6 |
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