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Sensitive and inexpensive digital DNA analysis by microfluidic enrichment of rolling circle amplified single-molecules

Single molecule quantification assays provide the ultimate sensitivity and precision for molecular analysis. However, most digital analysis techniques, i.e. droplet PCR, require sophisticated and expensive instrumentation for molecule compartmentalization, amplification and analysis. Rolling circle...

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Detalles Bibliográficos
Autores principales: Kühnemund, Malte, Hernández-Neuta, Iván, Sharif, Mohd Istiaq, Cornaglia, Matteo, Gijs, Martin A.M., Nilsson, Mats
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5416848/
https://www.ncbi.nlm.nih.gov/pubmed/28077562
http://dx.doi.org/10.1093/nar/gkw1324