EDTA aggregates induce SYPRO orange-based fluorescence in thermal shift assay

Ethylenediaminetetraacetic acid (EDTA) is widely used in the life sciences as chelating ligand of metal ions. However, formation of supramolecular EDTA aggregates at pH > 8 has been reported, which may lead to artifactual assay results. When applied as a buffer component at pH ≈ 10 in differentia...

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Detalles Bibliográficos
Autores principales: Kroeger, Tobias, Frieg, Benedikt, Zhang, Tao, Hansen, Finn K., Marmann, Andreas, Proksch, Peter, Nagel-Steger, Luitgard, Groth, Georg, Smits, Sander H. J., Gohlke, Holger
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5417642/
https://www.ncbi.nlm.nih.gov/pubmed/28472107
http://dx.doi.org/10.1371/journal.pone.0177024

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5417642/
https://www.ncbi.nlm.nih.gov/pubmed/28472107
http://dx.doi.org/10.1371/journal.pone.0177024

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