Cargando…

EDTA aggregates induce SYPRO orange-based fluorescence in thermal shift assay

Ethylenediaminetetraacetic acid (EDTA) is widely used in the life sciences as chelating ligand of metal ions. However, formation of supramolecular EDTA aggregates at pH > 8 has been reported, which may lead to artifactual assay results. When applied as a buffer component at pH ≈ 10 in differentia...

Descripción completa

Detalles Bibliográficos
Autores principales:	Kroeger, Tobias, Frieg, Benedikt, Zhang, Tao, Hansen, Finn K., Marmann, Andreas, Proksch, Peter, Nagel-Steger, Luitgard, Groth, Georg, Smits, Sander H. J., Gohlke, Holger
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2017
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5417642/ https://www.ncbi.nlm.nih.gov/pubmed/28472107 http://dx.doi.org/10.1371/journal.pone.0177024

Ejemplares similares

Biophysical and pharmacokinetic characterization of a small-molecule inhibitor of RUNX1/ETO tetramerization with anti-leukemic effects
por: Gopalswamy, Mohanraj, et al.
Publicado: (2022)

Latent Fingerprint Detection with SYPRO Rose Plus Protein Blot Stain
por: Bouldin, Kimberly K., et al.
Publicado: (2002)

Structural basis of lantibiotic recognition by the nisin resistance protein from Streptococcus agalactiae
por: Khosa, Sakshi, et al.
Publicado: (2016)

Met/Val129 polymorphism of the full-length human prion protein dictates distinct pathways of amyloid formation
por: Pauly, Thomas, et al.
Publicado: (2022)

Glutamine synthetase mutations that cause glutamine deficiency: mechanistic insights from molecular dynamics simulations
por: Frieg, Benedikt, et al.
Publicado: (2014)

Double-strand DNA end-binding and sliding of the toroidal CRISPR-associated protein Csn2
por: Arslan, Zihni, et al.
Publicado: (2013)

Atomic Resolution Insights into pH Shift Induced Deprotonation Events in LS-Shaped Aβ(1–42) Amyloid Fibrils
por: Becker, Nina, et al.
Publicado: (2023)

Human myocytes are protected from titin aggregation-induced stiffening by small heat shock proteins
por: Kötter, Sebastian, et al.
Publicado: (2014)

Molecular Mechanisms of Glutamine Synthetase Mutations that Lead to Clinically Relevant Pathologies
por: Frieg, Benedikt, et al.
Publicado: (2016)

Partially inserted nascent chain unzips the lateral gate of the Sec translocon
por: Kater, Lukas, et al.
Publicado: (2019)

Solution‐Based Determination of Dissociation Constants for the Binding of Aβ42 to Antibodies
por: Zhang, Tao, et al.
Publicado: (2019)

Co-Cultivation—A Powerful Emerging Tool for Enhancing the Chemical Diversity of Microorganisms
por: Marmann, Andreas, et al.
Publicado: (2014)

Aqueous ionic liquids redistribute local enzyme stability via long-range perturbation pathways
por: El Harrar, Till, et al.
Publicado: (2021)

Resolving Hot Spots in the C-Terminal Dimerization Domain that Determine the Stability of the Molecular Chaperone Hsp90
por: Ciglia, Emanuele, et al.
Publicado: (2014)

Interference with Amyloid-β Nucleation by Transient Ligand Interaction
por: Zhang, Tao, et al.
Publicado: (2019)

Amyloid β Oligomeric Species Present in the Lag Phase of Amyloid Formation
por: Wolff, Martin, et al.
Publicado: (2015)

Orange Fluorescent Proteins: Structural Studies of LSSmOrange, PSmOrange and PSmOrange2
por: Pletnev, Sergei, et al.
Publicado: (2014)

Immobilization of Homogeneous Monomeric, Oligomeric and Fibrillar Aβ Species for Reliable SPR Measurements
por: Frenzel, Daniel, et al.
Publicado: (2014)

Monomeric Amyloid Beta Peptide in Hexafluoroisopropanol Detected by Small Angle Neutron Scattering
por: Zhang-Haagen, Bo, et al.
Publicado: (2016)

Functional and structural characterization of interactions between opposite subunits in HCN pacemaker channels
por: Kondapuram, Mahesh, et al.
Publicado: (2022)

The ERA-EDTA today and tomorrow: a progress document by the ERA-EDTA Council
por: Zoccali, Carmine, et al.
Publicado: (2018)

Structure of the Response Regulator NsrR from Streptococcus agalactiae, Which Is Involved in Lantibiotic Resistance
por: Khosa, Sakshi, et al.
Publicado: (2016)

Next Congresses of the ERA-EDTA
Publicado: (2008)

Next Congresses of the ERA-EDTA
Publicado: (2010)

A photoswitchable orange-to-far-red fluorescent protein, PSmOrange
por: Subach, Oksana M., et al.
Publicado: (2011)

Dual-Mode Fluorescence and Visual Fluorescent Test Paper Detection of Copper Ions and EDTA
por: Xu, Wanqing, et al.
Publicado: (2021)

Molecular Interactions between Prions as Seeds and Recombinant Prion Proteins as Substrates Resemble the Biological Interspecies Barrier In Vitro
por: Panza, Giannantonio, et al.
Publicado: (2010)

The application of EDTA in drug delivery systems: doxorubicin liposomes loaded via NH(4)EDTA gradient
por: Song, Yanzhi, et al.
Publicado: (2014)

Biophysical Characterization of Nucleophosmin Interactions with Human Immunodeficiency Virus Rev and Herpes Simplex Virus US11
por: Nouri, Kazem, et al.
Publicado: (2015)

Shift Aggregate Extract Networks
por: Orsini, Francesco, et al.
Publicado: (2018)

ROLE OF EDTA AND METALS IN MITOCHONDRIAL CONTRACTION
por: Lynn, William S., et al.
Publicado: (1964)

Drawbacks of Dialysis Procedures for Removal of EDTA
por: Mónico, Andreia, et al.
Publicado: (2017)

EDTA Chelation Therapy for the Treatment of Neurotoxicity
por: Fulgenzi, Alessandro, et al.
Publicado: (2019)

Platelets–Leucocyte Satellitism: Love Is in the EDTA!
por: Lata, Bashundhara, et al.
Publicado: (2022)

Photoconversion in orange and red fluorescent proteins
por: Kremers, Gert-Jan, et al.
Publicado: (2009)

Seeded Fibrillation as Molecular Basis of the Species Barrier in Human Prion Diseases
por: Luers, Lars, et al.
Publicado: (2013)

Development of a First-in-Class Small-Molecule Inhibitor of the C-Terminal Hsp90 Dimerization
por: Bhatia, Sanil, et al.
Publicado: (2022)

Very bright orange fluorescent plants: endoplasmic reticulum targeting of orange fluorescent proteins as visual reporters in transgenic plants
por: Mann, David GJ, et al.
Publicado: (2012)

Protective effect of EDTA preadministration on renal ischemia
por: Foglieni, Chiara, et al.
Publicado: (2006)

Calcium and EDTA fluxes in dialyzed squid axons
Publicado: (1975)

Cannot write session to /tmp/vufind_sessions/sess_p25etvinleu6o077aqoi2tr0a0