A Novel and Efficient Method for Bacteria Genome Editing Employing both CRISPR/Cas9 and an Antibiotic Resistance Cassette

As Cas9-mediated cleavage requires both protospacer and protospacer adjacent motif (PAM) sequences, it is impossible to employ the CRISPR/Cas9 system to directly edit genomic sites without available PAM sequences nearby. Here, we optimized the CRISPR/Cas9 system and developed an innovative two-step...

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Detalles Bibliográficos
Autores principales: Zhang, Hong, Cheng, Qiu-Xiang, Liu, Ai-Min, Zhao, Guo-Ping, Wang, Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5418352/
https://www.ncbi.nlm.nih.gov/pubmed/28529507
http://dx.doi.org/10.3389/fmicb.2017.00812

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5418352/
https://www.ncbi.nlm.nih.gov/pubmed/28529507
http://dx.doi.org/10.3389/fmicb.2017.00812

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