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Relationship between recombinant protein expression and host metabolome as determined by two-dimensional NMR spectroscopy
Escherichia coli has been the most widely used host to produce large amounts of heterologous proteins. However, given an input plasmid DNA, E. coli may produce soluble protein, produce only inclusion bodies, or yield little or no protein at all. Many efforts have been made to surmount these problems...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2017
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5423636/ https://www.ncbi.nlm.nih.gov/pubmed/28486539 http://dx.doi.org/10.1371/journal.pone.0177233 |