Differences of microparticle patterns between sickle cell anemia and hemoglobin SC patients

Sickle cell anemia (SCA) and hemoglobin SC (HbSC) disease are the two most common forms of sickle cell disease (SCD), a frequent hemoglobinopathy which exhibits a highly variable clinical course. Although high levels of microparticles (MPs) have been consistently reported in SCA and evidence of thei...

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Autores principales: Garnier, Yohann, Ferdinand, Séverine, Etienne-Julan, Maryse, Elana, Gisèle, Petras, Marie, Doumdo, Lydia, Tressières, Benoit, Lalanne-Mistrih, Marie-Laure, Hardy-Dessources, Marie-Dominique, Connes, Philippe, Romana, Marc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5425024/
https://www.ncbi.nlm.nih.gov/pubmed/28489923
http://dx.doi.org/10.1371/journal.pone.0177397
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author Garnier, Yohann
Ferdinand, Séverine
Etienne-Julan, Maryse
Elana, Gisèle
Petras, Marie
Doumdo, Lydia
Tressières, Benoit
Lalanne-Mistrih, Marie-Laure
Hardy-Dessources, Marie-Dominique
Connes, Philippe
Romana, Marc
author_facet Garnier, Yohann
Ferdinand, Séverine
Etienne-Julan, Maryse
Elana, Gisèle
Petras, Marie
Doumdo, Lydia
Tressières, Benoit
Lalanne-Mistrih, Marie-Laure
Hardy-Dessources, Marie-Dominique
Connes, Philippe
Romana, Marc
author_sort Garnier, Yohann
collection PubMed
description Sickle cell anemia (SCA) and hemoglobin SC (HbSC) disease are the two most common forms of sickle cell disease (SCD), a frequent hemoglobinopathy which exhibits a highly variable clinical course. Although high levels of microparticles (MPs) have been consistently reported in SCA and evidence of their harmful impact on the SCA complication occurrences have been provided, no data on MP pattern in HbSC patients has been reported so far. In this study, we determined and compared the MP patterns of 84 HbSC and 96 SCA children, all at steady-state, using flow cytometry. Most of circulating MPs were derived from platelets (PLTs) and red blood cells (RBCs) in the two SCD syndromes. Moreover, we showed that HbSC patients exhibited lower blood concentration of total MPs compared to SCA patients, resulting mainly from a decrease of MP levels originated from RBCs and to a lesser extent from PLTs. We did not detect any association between blood MP concentrations and the occurrence of painful vaso-occlusive crises, acute chest syndrome and pulmonary hypertension in both patient groups. We also demonstrated for the first time, that whatever the considered genotype, RBC-derived MPs exhibited higher externalized phosphatidylserine level and were larger than PLT-derived MPs.
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spelling pubmed-54250242017-05-15 Differences of microparticle patterns between sickle cell anemia and hemoglobin SC patients Garnier, Yohann Ferdinand, Séverine Etienne-Julan, Maryse Elana, Gisèle Petras, Marie Doumdo, Lydia Tressières, Benoit Lalanne-Mistrih, Marie-Laure Hardy-Dessources, Marie-Dominique Connes, Philippe Romana, Marc PLoS One Research Article Sickle cell anemia (SCA) and hemoglobin SC (HbSC) disease are the two most common forms of sickle cell disease (SCD), a frequent hemoglobinopathy which exhibits a highly variable clinical course. Although high levels of microparticles (MPs) have been consistently reported in SCA and evidence of their harmful impact on the SCA complication occurrences have been provided, no data on MP pattern in HbSC patients has been reported so far. In this study, we determined and compared the MP patterns of 84 HbSC and 96 SCA children, all at steady-state, using flow cytometry. Most of circulating MPs were derived from platelets (PLTs) and red blood cells (RBCs) in the two SCD syndromes. Moreover, we showed that HbSC patients exhibited lower blood concentration of total MPs compared to SCA patients, resulting mainly from a decrease of MP levels originated from RBCs and to a lesser extent from PLTs. We did not detect any association between blood MP concentrations and the occurrence of painful vaso-occlusive crises, acute chest syndrome and pulmonary hypertension in both patient groups. We also demonstrated for the first time, that whatever the considered genotype, RBC-derived MPs exhibited higher externalized phosphatidylserine level and were larger than PLT-derived MPs. Public Library of Science 2017-05-10 /pmc/articles/PMC5425024/ /pubmed/28489923 http://dx.doi.org/10.1371/journal.pone.0177397 Text en © 2017 Garnier et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Garnier, Yohann
Ferdinand, Séverine
Etienne-Julan, Maryse
Elana, Gisèle
Petras, Marie
Doumdo, Lydia
Tressières, Benoit
Lalanne-Mistrih, Marie-Laure
Hardy-Dessources, Marie-Dominique
Connes, Philippe
Romana, Marc
Differences of microparticle patterns between sickle cell anemia and hemoglobin SC patients
title Differences of microparticle patterns between sickle cell anemia and hemoglobin SC patients
title_full Differences of microparticle patterns between sickle cell anemia and hemoglobin SC patients
title_fullStr Differences of microparticle patterns between sickle cell anemia and hemoglobin SC patients
title_full_unstemmed Differences of microparticle patterns between sickle cell anemia and hemoglobin SC patients
title_short Differences of microparticle patterns between sickle cell anemia and hemoglobin SC patients
title_sort differences of microparticle patterns between sickle cell anemia and hemoglobin sc patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5425024/
https://www.ncbi.nlm.nih.gov/pubmed/28489923
http://dx.doi.org/10.1371/journal.pone.0177397
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