Differences of microparticle patterns between sickle cell anemia and hemoglobin SC patients
Sickle cell anemia (SCA) and hemoglobin SC (HbSC) disease are the two most common forms of sickle cell disease (SCD), a frequent hemoglobinopathy which exhibits a highly variable clinical course. Although high levels of microparticles (MPs) have been consistently reported in SCA and evidence of thei...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5425024/ https://www.ncbi.nlm.nih.gov/pubmed/28489923 http://dx.doi.org/10.1371/journal.pone.0177397 |
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author | Garnier, Yohann Ferdinand, Séverine Etienne-Julan, Maryse Elana, Gisèle Petras, Marie Doumdo, Lydia Tressières, Benoit Lalanne-Mistrih, Marie-Laure Hardy-Dessources, Marie-Dominique Connes, Philippe Romana, Marc |
author_facet | Garnier, Yohann Ferdinand, Séverine Etienne-Julan, Maryse Elana, Gisèle Petras, Marie Doumdo, Lydia Tressières, Benoit Lalanne-Mistrih, Marie-Laure Hardy-Dessources, Marie-Dominique Connes, Philippe Romana, Marc |
author_sort | Garnier, Yohann |
collection | PubMed |
description | Sickle cell anemia (SCA) and hemoglobin SC (HbSC) disease are the two most common forms of sickle cell disease (SCD), a frequent hemoglobinopathy which exhibits a highly variable clinical course. Although high levels of microparticles (MPs) have been consistently reported in SCA and evidence of their harmful impact on the SCA complication occurrences have been provided, no data on MP pattern in HbSC patients has been reported so far. In this study, we determined and compared the MP patterns of 84 HbSC and 96 SCA children, all at steady-state, using flow cytometry. Most of circulating MPs were derived from platelets (PLTs) and red blood cells (RBCs) in the two SCD syndromes. Moreover, we showed that HbSC patients exhibited lower blood concentration of total MPs compared to SCA patients, resulting mainly from a decrease of MP levels originated from RBCs and to a lesser extent from PLTs. We did not detect any association between blood MP concentrations and the occurrence of painful vaso-occlusive crises, acute chest syndrome and pulmonary hypertension in both patient groups. We also demonstrated for the first time, that whatever the considered genotype, RBC-derived MPs exhibited higher externalized phosphatidylserine level and were larger than PLT-derived MPs. |
format | Online Article Text |
id | pubmed-5425024 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-54250242017-05-15 Differences of microparticle patterns between sickle cell anemia and hemoglobin SC patients Garnier, Yohann Ferdinand, Séverine Etienne-Julan, Maryse Elana, Gisèle Petras, Marie Doumdo, Lydia Tressières, Benoit Lalanne-Mistrih, Marie-Laure Hardy-Dessources, Marie-Dominique Connes, Philippe Romana, Marc PLoS One Research Article Sickle cell anemia (SCA) and hemoglobin SC (HbSC) disease are the two most common forms of sickle cell disease (SCD), a frequent hemoglobinopathy which exhibits a highly variable clinical course. Although high levels of microparticles (MPs) have been consistently reported in SCA and evidence of their harmful impact on the SCA complication occurrences have been provided, no data on MP pattern in HbSC patients has been reported so far. In this study, we determined and compared the MP patterns of 84 HbSC and 96 SCA children, all at steady-state, using flow cytometry. Most of circulating MPs were derived from platelets (PLTs) and red blood cells (RBCs) in the two SCD syndromes. Moreover, we showed that HbSC patients exhibited lower blood concentration of total MPs compared to SCA patients, resulting mainly from a decrease of MP levels originated from RBCs and to a lesser extent from PLTs. We did not detect any association between blood MP concentrations and the occurrence of painful vaso-occlusive crises, acute chest syndrome and pulmonary hypertension in both patient groups. We also demonstrated for the first time, that whatever the considered genotype, RBC-derived MPs exhibited higher externalized phosphatidylserine level and were larger than PLT-derived MPs. Public Library of Science 2017-05-10 /pmc/articles/PMC5425024/ /pubmed/28489923 http://dx.doi.org/10.1371/journal.pone.0177397 Text en © 2017 Garnier et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Garnier, Yohann Ferdinand, Séverine Etienne-Julan, Maryse Elana, Gisèle Petras, Marie Doumdo, Lydia Tressières, Benoit Lalanne-Mistrih, Marie-Laure Hardy-Dessources, Marie-Dominique Connes, Philippe Romana, Marc Differences of microparticle patterns between sickle cell anemia and hemoglobin SC patients |
title | Differences of microparticle patterns between sickle cell anemia and hemoglobin SC patients |
title_full | Differences of microparticle patterns between sickle cell anemia and hemoglobin SC patients |
title_fullStr | Differences of microparticle patterns between sickle cell anemia and hemoglobin SC patients |
title_full_unstemmed | Differences of microparticle patterns between sickle cell anemia and hemoglobin SC patients |
title_short | Differences of microparticle patterns between sickle cell anemia and hemoglobin SC patients |
title_sort | differences of microparticle patterns between sickle cell anemia and hemoglobin sc patients |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5425024/ https://www.ncbi.nlm.nih.gov/pubmed/28489923 http://dx.doi.org/10.1371/journal.pone.0177397 |
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