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RhoD Inhibits RhoC-ROCK-Dependent Cell Contraction via PAK6

RhoA-mediated regulation of myosin-II activity in the actin cortex controls the ability of cells to contract and bleb during a variety of cellular processes, including cell migration and division. Cell contraction and blebbing also frequently occur as part of the cytopathic effect seen during many d...

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Autores principales: Durkin, Charlotte H., Leite, Flavia, Cordeiro, João V., Handa, Yutaka, Arakawa, Yoshiki, Valderrama, Ferran, Way, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5425256/
https://www.ncbi.nlm.nih.gov/pubmed/28486133
http://dx.doi.org/10.1016/j.devcel.2017.04.010
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author Durkin, Charlotte H.
Leite, Flavia
Cordeiro, João V.
Handa, Yutaka
Arakawa, Yoshiki
Valderrama, Ferran
Way, Michael
author_facet Durkin, Charlotte H.
Leite, Flavia
Cordeiro, João V.
Handa, Yutaka
Arakawa, Yoshiki
Valderrama, Ferran
Way, Michael
author_sort Durkin, Charlotte H.
collection PubMed
description RhoA-mediated regulation of myosin-II activity in the actin cortex controls the ability of cells to contract and bleb during a variety of cellular processes, including cell migration and division. Cell contraction and blebbing also frequently occur as part of the cytopathic effect seen during many different viral infections. We now demonstrate that the vaccinia virus protein F11, which localizes to the plasma membrane, is required for ROCK-mediated cell contraction from 2 hr post infection. Curiously, F11-induced cell contraction is dependent on RhoC and not RhoA signaling to ROCK. Moreover, RhoC-driven cell contraction depends on the upstream inhibition of RhoD signaling by F11. This inhibition prevents RhoD from regulating its downstream effector Pak6, alleviating the suppression of RhoC by the kinase. Our observations with vaccinia have now demonstrated that RhoD recruits Pak6 to the plasma membrane to antagonize RhoC signaling during cell contraction and blebbing.
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spelling pubmed-54252562017-05-11 RhoD Inhibits RhoC-ROCK-Dependent Cell Contraction via PAK6 Durkin, Charlotte H. Leite, Flavia Cordeiro, João V. Handa, Yutaka Arakawa, Yoshiki Valderrama, Ferran Way, Michael Dev Cell Article RhoA-mediated regulation of myosin-II activity in the actin cortex controls the ability of cells to contract and bleb during a variety of cellular processes, including cell migration and division. Cell contraction and blebbing also frequently occur as part of the cytopathic effect seen during many different viral infections. We now demonstrate that the vaccinia virus protein F11, which localizes to the plasma membrane, is required for ROCK-mediated cell contraction from 2 hr post infection. Curiously, F11-induced cell contraction is dependent on RhoC and not RhoA signaling to ROCK. Moreover, RhoC-driven cell contraction depends on the upstream inhibition of RhoD signaling by F11. This inhibition prevents RhoD from regulating its downstream effector Pak6, alleviating the suppression of RhoC by the kinase. Our observations with vaccinia have now demonstrated that RhoD recruits Pak6 to the plasma membrane to antagonize RhoC signaling during cell contraction and blebbing. Cell Press 2017-05-08 /pmc/articles/PMC5425256/ /pubmed/28486133 http://dx.doi.org/10.1016/j.devcel.2017.04.010 Text en © 2017 The Francis Crick Institute http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Durkin, Charlotte H.
Leite, Flavia
Cordeiro, João V.
Handa, Yutaka
Arakawa, Yoshiki
Valderrama, Ferran
Way, Michael
RhoD Inhibits RhoC-ROCK-Dependent Cell Contraction via PAK6
title RhoD Inhibits RhoC-ROCK-Dependent Cell Contraction via PAK6
title_full RhoD Inhibits RhoC-ROCK-Dependent Cell Contraction via PAK6
title_fullStr RhoD Inhibits RhoC-ROCK-Dependent Cell Contraction via PAK6
title_full_unstemmed RhoD Inhibits RhoC-ROCK-Dependent Cell Contraction via PAK6
title_short RhoD Inhibits RhoC-ROCK-Dependent Cell Contraction via PAK6
title_sort rhod inhibits rhoc-rock-dependent cell contraction via pak6
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5425256/
https://www.ncbi.nlm.nih.gov/pubmed/28486133
http://dx.doi.org/10.1016/j.devcel.2017.04.010
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