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RhoD Inhibits RhoC-ROCK-Dependent Cell Contraction via PAK6
RhoA-mediated regulation of myosin-II activity in the actin cortex controls the ability of cells to contract and bleb during a variety of cellular processes, including cell migration and division. Cell contraction and blebbing also frequently occur as part of the cytopathic effect seen during many d...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cell Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5425256/ https://www.ncbi.nlm.nih.gov/pubmed/28486133 http://dx.doi.org/10.1016/j.devcel.2017.04.010 |
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author | Durkin, Charlotte H. Leite, Flavia Cordeiro, João V. Handa, Yutaka Arakawa, Yoshiki Valderrama, Ferran Way, Michael |
author_facet | Durkin, Charlotte H. Leite, Flavia Cordeiro, João V. Handa, Yutaka Arakawa, Yoshiki Valderrama, Ferran Way, Michael |
author_sort | Durkin, Charlotte H. |
collection | PubMed |
description | RhoA-mediated regulation of myosin-II activity in the actin cortex controls the ability of cells to contract and bleb during a variety of cellular processes, including cell migration and division. Cell contraction and blebbing also frequently occur as part of the cytopathic effect seen during many different viral infections. We now demonstrate that the vaccinia virus protein F11, which localizes to the plasma membrane, is required for ROCK-mediated cell contraction from 2 hr post infection. Curiously, F11-induced cell contraction is dependent on RhoC and not RhoA signaling to ROCK. Moreover, RhoC-driven cell contraction depends on the upstream inhibition of RhoD signaling by F11. This inhibition prevents RhoD from regulating its downstream effector Pak6, alleviating the suppression of RhoC by the kinase. Our observations with vaccinia have now demonstrated that RhoD recruits Pak6 to the plasma membrane to antagonize RhoC signaling during cell contraction and blebbing. |
format | Online Article Text |
id | pubmed-5425256 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Cell Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-54252562017-05-11 RhoD Inhibits RhoC-ROCK-Dependent Cell Contraction via PAK6 Durkin, Charlotte H. Leite, Flavia Cordeiro, João V. Handa, Yutaka Arakawa, Yoshiki Valderrama, Ferran Way, Michael Dev Cell Article RhoA-mediated regulation of myosin-II activity in the actin cortex controls the ability of cells to contract and bleb during a variety of cellular processes, including cell migration and division. Cell contraction and blebbing also frequently occur as part of the cytopathic effect seen during many different viral infections. We now demonstrate that the vaccinia virus protein F11, which localizes to the plasma membrane, is required for ROCK-mediated cell contraction from 2 hr post infection. Curiously, F11-induced cell contraction is dependent on RhoC and not RhoA signaling to ROCK. Moreover, RhoC-driven cell contraction depends on the upstream inhibition of RhoD signaling by F11. This inhibition prevents RhoD from regulating its downstream effector Pak6, alleviating the suppression of RhoC by the kinase. Our observations with vaccinia have now demonstrated that RhoD recruits Pak6 to the plasma membrane to antagonize RhoC signaling during cell contraction and blebbing. Cell Press 2017-05-08 /pmc/articles/PMC5425256/ /pubmed/28486133 http://dx.doi.org/10.1016/j.devcel.2017.04.010 Text en © 2017 The Francis Crick Institute http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Durkin, Charlotte H. Leite, Flavia Cordeiro, João V. Handa, Yutaka Arakawa, Yoshiki Valderrama, Ferran Way, Michael RhoD Inhibits RhoC-ROCK-Dependent Cell Contraction via PAK6 |
title | RhoD Inhibits RhoC-ROCK-Dependent Cell Contraction via PAK6 |
title_full | RhoD Inhibits RhoC-ROCK-Dependent Cell Contraction via PAK6 |
title_fullStr | RhoD Inhibits RhoC-ROCK-Dependent Cell Contraction via PAK6 |
title_full_unstemmed | RhoD Inhibits RhoC-ROCK-Dependent Cell Contraction via PAK6 |
title_short | RhoD Inhibits RhoC-ROCK-Dependent Cell Contraction via PAK6 |
title_sort | rhod inhibits rhoc-rock-dependent cell contraction via pak6 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5425256/ https://www.ncbi.nlm.nih.gov/pubmed/28486133 http://dx.doi.org/10.1016/j.devcel.2017.04.010 |
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