Single-Cell Dynamic Analysis of Mitosis in Haploid Embryonic Stem Cells Shows the Prolonged Metaphase and Its Association with Self-diploidization

The recent establishment of mammalian haploid embryonic stem cells (ESCs) provides new possibilities for genetic screening and for understanding genome evolution and function. However, the dynamics of mitosis in haploid ESCs is still unclear. Here, we report that the duration of mitosis in haploid E...

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Autores principales: Guo, Ao, Huang, Shichao, Yu, Jiali, Wang, Huihan, Li, Haisen, Pei, Gang, Shen, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5425685/
https://www.ncbi.nlm.nih.gov/pubmed/28457886
http://dx.doi.org/10.1016/j.stemcr.2017.03.025
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author Guo, Ao
Huang, Shichao
Yu, Jiali
Wang, Huihan
Li, Haisen
Pei, Gang
Shen, Li
author_facet Guo, Ao
Huang, Shichao
Yu, Jiali
Wang, Huihan
Li, Haisen
Pei, Gang
Shen, Li
author_sort Guo, Ao
collection PubMed
description The recent establishment of mammalian haploid embryonic stem cells (ESCs) provides new possibilities for genetic screening and for understanding genome evolution and function. However, the dynamics of mitosis in haploid ESCs is still unclear. Here, we report that the duration of mitosis in haploid ESCs, especially the metaphase, is significantly longer than that in diploid ESCs. Delaying mitosis by chemicals increased self-diploidization of haploid ESCs, while shortening mitosis stabilized haploid ESCs. Taken together, our study suggests that the delayed mitosis of haploid ESCs is associated with self-diploidization.
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spelling pubmed-54256852017-05-17 Single-Cell Dynamic Analysis of Mitosis in Haploid Embryonic Stem Cells Shows the Prolonged Metaphase and Its Association with Self-diploidization Guo, Ao Huang, Shichao Yu, Jiali Wang, Huihan Li, Haisen Pei, Gang Shen, Li Stem Cell Reports Report The recent establishment of mammalian haploid embryonic stem cells (ESCs) provides new possibilities for genetic screening and for understanding genome evolution and function. However, the dynamics of mitosis in haploid ESCs is still unclear. Here, we report that the duration of mitosis in haploid ESCs, especially the metaphase, is significantly longer than that in diploid ESCs. Delaying mitosis by chemicals increased self-diploidization of haploid ESCs, while shortening mitosis stabilized haploid ESCs. Taken together, our study suggests that the delayed mitosis of haploid ESCs is associated with self-diploidization. Elsevier 2017-04-27 /pmc/articles/PMC5425685/ /pubmed/28457886 http://dx.doi.org/10.1016/j.stemcr.2017.03.025 Text en © 2017 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Report
Guo, Ao
Huang, Shichao
Yu, Jiali
Wang, Huihan
Li, Haisen
Pei, Gang
Shen, Li
Single-Cell Dynamic Analysis of Mitosis in Haploid Embryonic Stem Cells Shows the Prolonged Metaphase and Its Association with Self-diploidization
title Single-Cell Dynamic Analysis of Mitosis in Haploid Embryonic Stem Cells Shows the Prolonged Metaphase and Its Association with Self-diploidization
title_full Single-Cell Dynamic Analysis of Mitosis in Haploid Embryonic Stem Cells Shows the Prolonged Metaphase and Its Association with Self-diploidization
title_fullStr Single-Cell Dynamic Analysis of Mitosis in Haploid Embryonic Stem Cells Shows the Prolonged Metaphase and Its Association with Self-diploidization
title_full_unstemmed Single-Cell Dynamic Analysis of Mitosis in Haploid Embryonic Stem Cells Shows the Prolonged Metaphase and Its Association with Self-diploidization
title_short Single-Cell Dynamic Analysis of Mitosis in Haploid Embryonic Stem Cells Shows the Prolonged Metaphase and Its Association with Self-diploidization
title_sort single-cell dynamic analysis of mitosis in haploid embryonic stem cells shows the prolonged metaphase and its association with self-diploidization
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5425685/
https://www.ncbi.nlm.nih.gov/pubmed/28457886
http://dx.doi.org/10.1016/j.stemcr.2017.03.025
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