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Spectral imaging toolbox: segmentation, hyperstack reconstruction, and batch processing of spectral images for the determination of cell and model membrane lipid order

BACKGROUND: Spectral imaging with polarity-sensitive fluorescent probes enables the quantification of cell and model membrane physical properties, including local hydration, fluidity, and lateral lipid packing, usually characterized by the generalized polarization (GP) parameter. With the developmen...

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Detalles Bibliográficos
Autores principales: Aron, Miles, Browning, Richard, Carugo, Dario, Sezgin, Erdinc, Bernardino de la Serna, Jorge, Eggeling, Christian, Stride, Eleanor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5427590/
https://www.ncbi.nlm.nih.gov/pubmed/28494801
http://dx.doi.org/10.1186/s12859-017-1656-2