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Spectral imaging toolbox: segmentation, hyperstack reconstruction, and batch processing of spectral images for the determination of cell and model membrane lipid order
BACKGROUND: Spectral imaging with polarity-sensitive fluorescent probes enables the quantification of cell and model membrane physical properties, including local hydration, fluidity, and lateral lipid packing, usually characterized by the generalized polarization (GP) parameter. With the developmen...
Autores principales: | Aron, Miles, Browning, Richard, Carugo, Dario, Sezgin, Erdinc, Bernardino de la Serna, Jorge, Eggeling, Christian, Stride, Eleanor |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5427590/ https://www.ncbi.nlm.nih.gov/pubmed/28494801 http://dx.doi.org/10.1186/s12859-017-1656-2 |
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