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New platform for simple and rapid protein-based affinity reactions

We developed a spongy-like porous polymer (spongy monolith) consisting of poly(ethylene-co-glycidyl methacrylate) with continuous macropores that allowed efficient in situ reaction between the epoxy groups and proteins of interest. Immobilization of protein A on the spongy monolith enabled high-yiel...

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Detalles Bibliográficos
Autores principales: Kubota, Kei, Kubo, Takuya, Tanigawa, Tetsuya, Naito, Toyohiro, Otsuka, Koji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428043/
https://www.ncbi.nlm.nih.gov/pubmed/28282970
http://dx.doi.org/10.1038/s41598-017-00264-y