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New platform for simple and rapid protein-based affinity reactions
We developed a spongy-like porous polymer (spongy monolith) consisting of poly(ethylene-co-glycidyl methacrylate) with continuous macropores that allowed efficient in situ reaction between the epoxy groups and proteins of interest. Immobilization of protein A on the spongy monolith enabled high-yiel...
Autores principales: | Kubota, Kei, Kubo, Takuya, Tanigawa, Tetsuya, Naito, Toyohiro, Otsuka, Koji |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428043/ https://www.ncbi.nlm.nih.gov/pubmed/28282970 http://dx.doi.org/10.1038/s41598-017-00264-y |
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