Co-expression of BirA with biotin bait achieves in vivo biotinylation of overexpressed stable N-glycosylated sRAGE in transgenic silkworms

Here, we demonstrated the expression of the N-glycosylated extracellular ligand binding domain of receptor for advanced glycation end products (sRAGE) in middle silk glands (MSGs) of transgenic silkworms using the GAL4/UAS system. Over 1 mg of sRAGE was obtained from one transgenic silkworm. sRAGE p...

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Detalles Bibliográficos
Autores principales: Kumano-Kuramochi, Miyuki, Tatematsu, Ken-ichiro, Ohnishi-Kameyama, Mayumi, Maeda-Yamamoto, Mari, Kobori, Toshiro, Sezutsu, Hideki, Machida, Sachiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428419/
https://www.ncbi.nlm.nih.gov/pubmed/28336960
http://dx.doi.org/10.1038/s41598-017-00420-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428419/
https://www.ncbi.nlm.nih.gov/pubmed/28336960
http://dx.doi.org/10.1038/s41598-017-00420-4

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