Cargando…

A Convenient Cas9-based Conditional Knockout Strategy for Simultaneously Targeting Multiple Genes in Mouse

The most powerful way to probe protein function is to characterize the consequence of its deletion. Compared to conventional gene knockout (KO), conditional knockout (cKO) provides an advanced gene targeting strategy with which gene deletion can be performed in a spatially and temporally restricted...

Descripción completa

Detalles Bibliográficos
Autores principales:	Chen, Jiang, Du, Yinan, He, Xueyan, Huang, Xingxu, Shi, Yun S.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428785/ https://www.ncbi.nlm.nih.gov/pubmed/28364122 http://dx.doi.org/10.1038/s41598-017-00654-2

Ejemplares similares

User-Friendly Genetic Conditional Knockout Strategies by CRISPR/Cas9
por: Chen, Liangliang, et al.
Publicado: (2018)

Generation of an Oocyte-Specific Cas9 Transgenic Mouse for Genome Editing
por: Zhang, Linlin, et al.
Publicado: (2016)

CRISPR/Cas9-mediated gene knockout in the mouse brain using in utero electroporation
por: Shinmyo, Yohei, et al.
Publicado: (2016)

CRISPR/Cas9-mediated generation of a Plac8 knockout mouse model
por: Lee, HyunJeong, et al.
Publicado: (2018)

CRISPR/Cas9-mediated knockout of intracellular molecule SHP-1 enhances tumor-killing ability of CD133-targeted CAR T cells in vitro
por: Liu, Ming, et al.
Publicado: (2023)

CRISPR-Cas9-induced gene knockout in zebrafish
por: Medishetti, Raghavender, et al.
Publicado: (2022)

An improved strategy for CRISPR/Cas9 gene knockout and subsequent wildtype and mutant gene rescue
por: Jin, Jiankang, et al.
Publicado: (2020)

Rapid generation of conditional knockout mice using the CRISPR-Cas9 system and electroporation for neuroscience research
por: Nishizono, Hirofumi, et al.
Publicado: (2021)

One-step CRISPR-Cas9 protocol for the generation of plug & play conditional knockouts in Drosophila melanogaster
por: Yu, Joyce J.S., et al.
Publicado: (2021)

Simultaneous knockout of multiple LHCF genes using single sgRNAs and engineering of a high‐fidelity Cas9 for precise genome editing in marine algae
por: Sharma, Amit K., et al.
Publicado: (2021)

Generation of mouse hippocampal neural precursor cell lines with CRISPR/Cas9-mediated gene knockouts
por: Zocher, Sara, et al.
Publicado: (2021)

JACKS: joint analysis of CRISPR/Cas9 knockout screens
por: Allen, Felicity, et al.
Publicado: (2019)

CRISPR/Cas9-induced knockout and knock-in mutations in Chlamydomonas reinhardtii
por: Shin, Sung-Eun, et al.
Publicado: (2016)

Generation of Gene-Knockout Mongolian Gerbils via CRISPR/Cas9 System
por: Wang, Yan, et al.
Publicado: (2020)

Construction and Evaluation of a Novel Organic Anion Transporter 1/3 CRISPR/Cas9 Double-Knockout Rat Model
por: Gou, Xueyan, et al.
Publicado: (2022)

CRISPR/Cas9 Knockout Strategies to Ablate CCAT1 lncRNA Gene in Cancer Cells
por: Zare, Khadijeh, et al.
Publicado: (2018)

The Convenience of Single Homology Arm Donor DNA and CRISPR/Cas9-Nickase for Targeted Insertion of Long DNA Fragment
por: Basiri, Mohsen, et al.
Publicado: (2017)

In Vivo Knockout of the Vegfa Gene by Lentiviral Delivery of CRISPR/Cas9 in Mouse Retinal Pigment Epithelium Cells
por: Holmgaard, Andreas, et al.
Publicado: (2017)

Efficient Production of Biallelic RAG1 Knockout Mouse Embryonic Stem Cell Using CRISPR/Cas9
por: Mehravar, Maryam, et al.
Publicado: (2019)

Multigene Knockout Utilizing Off-Target Mutations of the CRISPR/Cas9 System in Rice
por: Endo, Masaki, et al.
Publicado: (2015)

Efficient Gene Knockout in Goats Using CRISPR/Cas9 System
por: Ni, Wei, et al.
Publicado: (2014)

CRISPR/Cas9-mediated gene knockout in the ascidian Ciona intestinalis
por: Sasaki, Haruka, et al.
Publicado: (2014)

CRISPR/Cas9 Methodology for the Generation of Knockout Deletions in Caenorhabditis elegans
por: Au, Vinci, et al.
Publicado: (2018)

Consequences of CRISPR-Cas9-Mediated CFTR Knockout in Human Macrophages
por: Zhang, Shuzhong, et al.
Publicado: (2020)

Simplified Gene Knockout by CRISPR-Cas9-Induced Homologous Recombination
por: Dalvie, Neil C., et al.
Publicado: (2021)

The efficient generation of knockout microglia cells using a dual-sgRNA strategy by CRISPR/Cas9
por: Zhang, Mengfei, et al.
Publicado: (2022)

Efficient generation of gene-modified pigs via injection of zygote with Cas9/sgRNA
por: Wang, Yong, et al.
Publicado: (2015)

One-Step Genotyping Method in loxP-Based Conditional Knockout Mice Generated by CRISPR-Cas9 Technology
por: Zhu, He, et al.
Publicado: (2022)

Characterization of an Osmr Conditional Knockout Mouse Model
por: Schwartz, Logan S., et al.
Publicado: (2023)

Immunoblot screening of CRISPR/Cas9-mediated gene knockouts without selection
por: Estep, Jason A., et al.
Publicado: (2016)

CRISPR-Cas9-Based Knockout of the Prion Protein and Its Effect on the Proteome
por: Mehrabian, Mohadeseh, et al.
Publicado: (2014)

Optimizing sgRNA structure to improve CRISPR-Cas9 knockout efficiency
por: Dang, Ying, et al.
Publicado: (2015)

Evaluation and Design of Genome-Wide CRISPR/SpCas9 Knockout Screens
por: Hart, Traver, et al.
Publicado: (2017)

Efficient knockout of phytoene desaturase gene using CRISPR/Cas9 in melon
por: Hooghvorst, Isidre, et al.
Publicado: (2019)

An efficient miRNA knockout approach using CRISPR-Cas9 in Xenopus()
por: Godden, Alice M., et al.
Publicado: (2022)

Knockout of phytoene desaturase gene using CRISPR/Cas9 in highbush blueberry
por: Vaia, Giuseppe, et al.
Publicado: (2022)

Generation of gene-of-interest knockouts in murine organoids using CRISPR-Cas9
por: Huber, Anne, et al.
Publicado: (2023)

Impact of CRISPR/Cas9-Mediated CD73 Knockout in Pancreatic Cancer
por: Zhang, Jinping, et al.
Publicado: (2023)

CRISPR-Cas9 knockout screening identifies KIAA1429 as an essential gene in Ewing sarcoma
por: Tan, Kezhe, et al.
Publicado: (2023)

CRISPR-Cas9 mediated efficient PD-1 disruption on human primary T cells from cancer patients
por: Su, Shu, et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_8oa0cb2qgdkirs39m7fljl4bk0