Cargando…

Pentamethylquercetin induces adipose browning and exerts beneficial effects in 3T3-L1 adipocytes and high-fat diet-fed mice

Browning white adipocytes may be a new target in anti-obesity therapy. Pentamethylquercetin (PMQ) has been shown to have anti-obesity effects in monosodium glutamate-induced obese mice. Here, we aimed to study the anti-obesity effects of PMQ in vitro and in vivo and to determine if adipose browning...

Descripción completa

Detalles Bibliográficos
Autores principales: Han, Yi, Wu, Jian-Zhao, Shen, Ji-zhong, Chen, Lei, He, Ting, Jin, Man-wen, Liu, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5430711/
https://www.ncbi.nlm.nih.gov/pubmed/28442748
http://dx.doi.org/10.1038/s41598-017-01206-4
_version_ 1783236277226700800
author Han, Yi
Wu, Jian-Zhao
Shen, Ji-zhong
Chen, Lei
He, Ting
Jin, Man-wen
Liu, Hui
author_facet Han, Yi
Wu, Jian-Zhao
Shen, Ji-zhong
Chen, Lei
He, Ting
Jin, Man-wen
Liu, Hui
author_sort Han, Yi
collection PubMed
description Browning white adipocytes may be a new target in anti-obesity therapy. Pentamethylquercetin (PMQ) has been shown to have anti-obesity effects in monosodium glutamate-induced obese mice. Here, we aimed to study the anti-obesity effects of PMQ in vitro and in vivo and to determine if adipose browning is involved in the mechanism underlying the anti-obesity effects of PMQ. We evaluated the effects of PMQ on cell proliferation, cell differentiation, glucose consumption, cellular lipid metabolism, and related brown gene expression in 3T3-L1 adipocytes. We also investigated the effects of PMQ in a mouse model of high-fat diet (HFD)-induced obesity. Our results demonstrated that PMQ increased the consumption of glucose, inhibited the accumulation of cellular triglycerides (TGs), and induced the expression of brown adipocyte-specific genes, such as uncoupling protein 1 (UCP-1), during the early stage of differentiation in 3T3-L1 adipocytes. In HFD mice, PMQ treatment reduced waist circumference, LEE index, white adipose tissue (WAT) weight and white adipocyte size and increased brown adipose tissue (BAT) weight. Moreover, PMQ treatment induced mitochondrial biogenesis and upregulated UCP-1 expression in WAT. These findings suggest that PMQ may induce browning of adipose tissue, a phenomenon that is at least partly related to its anti-obesity effects.
format Online
Article
Text
id pubmed-5430711
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-54307112017-05-16 Pentamethylquercetin induces adipose browning and exerts beneficial effects in 3T3-L1 adipocytes and high-fat diet-fed mice Han, Yi Wu, Jian-Zhao Shen, Ji-zhong Chen, Lei He, Ting Jin, Man-wen Liu, Hui Sci Rep Article Browning white adipocytes may be a new target in anti-obesity therapy. Pentamethylquercetin (PMQ) has been shown to have anti-obesity effects in monosodium glutamate-induced obese mice. Here, we aimed to study the anti-obesity effects of PMQ in vitro and in vivo and to determine if adipose browning is involved in the mechanism underlying the anti-obesity effects of PMQ. We evaluated the effects of PMQ on cell proliferation, cell differentiation, glucose consumption, cellular lipid metabolism, and related brown gene expression in 3T3-L1 adipocytes. We also investigated the effects of PMQ in a mouse model of high-fat diet (HFD)-induced obesity. Our results demonstrated that PMQ increased the consumption of glucose, inhibited the accumulation of cellular triglycerides (TGs), and induced the expression of brown adipocyte-specific genes, such as uncoupling protein 1 (UCP-1), during the early stage of differentiation in 3T3-L1 adipocytes. In HFD mice, PMQ treatment reduced waist circumference, LEE index, white adipose tissue (WAT) weight and white adipocyte size and increased brown adipose tissue (BAT) weight. Moreover, PMQ treatment induced mitochondrial biogenesis and upregulated UCP-1 expression in WAT. These findings suggest that PMQ may induce browning of adipose tissue, a phenomenon that is at least partly related to its anti-obesity effects. Nature Publishing Group UK 2017-04-25 /pmc/articles/PMC5430711/ /pubmed/28442748 http://dx.doi.org/10.1038/s41598-017-01206-4 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Han, Yi
Wu, Jian-Zhao
Shen, Ji-zhong
Chen, Lei
He, Ting
Jin, Man-wen
Liu, Hui
Pentamethylquercetin induces adipose browning and exerts beneficial effects in 3T3-L1 adipocytes and high-fat diet-fed mice
title Pentamethylquercetin induces adipose browning and exerts beneficial effects in 3T3-L1 adipocytes and high-fat diet-fed mice
title_full Pentamethylquercetin induces adipose browning and exerts beneficial effects in 3T3-L1 adipocytes and high-fat diet-fed mice
title_fullStr Pentamethylquercetin induces adipose browning and exerts beneficial effects in 3T3-L1 adipocytes and high-fat diet-fed mice
title_full_unstemmed Pentamethylquercetin induces adipose browning and exerts beneficial effects in 3T3-L1 adipocytes and high-fat diet-fed mice
title_short Pentamethylquercetin induces adipose browning and exerts beneficial effects in 3T3-L1 adipocytes and high-fat diet-fed mice
title_sort pentamethylquercetin induces adipose browning and exerts beneficial effects in 3t3-l1 adipocytes and high-fat diet-fed mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5430711/
https://www.ncbi.nlm.nih.gov/pubmed/28442748
http://dx.doi.org/10.1038/s41598-017-01206-4
work_keys_str_mv AT hanyi pentamethylquercetininducesadiposebrowningandexertsbeneficialeffectsin3t3l1adipocytesandhighfatdietfedmice
AT wujianzhao pentamethylquercetininducesadiposebrowningandexertsbeneficialeffectsin3t3l1adipocytesandhighfatdietfedmice
AT shenjizhong pentamethylquercetininducesadiposebrowningandexertsbeneficialeffectsin3t3l1adipocytesandhighfatdietfedmice
AT chenlei pentamethylquercetininducesadiposebrowningandexertsbeneficialeffectsin3t3l1adipocytesandhighfatdietfedmice
AT heting pentamethylquercetininducesadiposebrowningandexertsbeneficialeffectsin3t3l1adipocytesandhighfatdietfedmice
AT jinmanwen pentamethylquercetininducesadiposebrowningandexertsbeneficialeffectsin3t3l1adipocytesandhighfatdietfedmice
AT liuhui pentamethylquercetininducesadiposebrowningandexertsbeneficialeffectsin3t3l1adipocytesandhighfatdietfedmice