Agarose Spot as a Comparative Method for in situ Analysis of Simultaneous Chemotactic Responses to Multiple Chemokines

We describe a novel protocol to quantitatively and simultaneously compare the chemotactic responses of cells towards different chemokines. In this protocol, droplets of agarose gel containing different chemokines are applied onto the surface of a Petri dish, and then immersed under culture medium in...

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Detalles Bibliográficos
Autores principales: Ahmed, Mohaned, Basheer, Haneen A., Ayuso, Jose M., Ahmet, Djevdet, Mazzini, Marco, Patel, Roshan, Shnyder, Steven D., Vinader, Victoria, Afarinkia, Kamyar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5430824/
https://www.ncbi.nlm.nih.gov/pubmed/28432337
http://dx.doi.org/10.1038/s41598-017-00949-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5430824/
https://www.ncbi.nlm.nih.gov/pubmed/28432337
http://dx.doi.org/10.1038/s41598-017-00949-4

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