Enhancing the throughput and multiplexing capabilities of next generation sequencing for efficient implementation of pooled shRNA and CRISPR screens

Next generation sequencing is becoming the method of choice for functional genomic studies that use pooled shRNA or CRISPR libraries. A key challenge in sequencing these mixed-oligo libraries is that they are highly susceptible to hairpin and/or heteroduplex formation. This results in polyclonal, lo...

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Detalles Bibliográficos
Autores principales: Islam, Md. Fahmid, Watanabe, Atsushi, Wong, Lai, Lazarou, Conor, Vizeacoumar, Frederick S., Abuhussein, Omar, Hill, Wayne, Uppalapati, Maruti, Geyer, C. Ronald, Vizeacoumar, Franco J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5430825/
https://www.ncbi.nlm.nih.gov/pubmed/28432350
http://dx.doi.org/10.1038/s41598-017-01170-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5430825/
https://www.ncbi.nlm.nih.gov/pubmed/28432350
http://dx.doi.org/10.1038/s41598-017-01170-z

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