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N-Glycan Modification of a Recombinant Protein via Coexpression of Human Glycosyltransferases in Silkworm Pupae

Recombinant proteins produced in insect cells and insects, unlike those produced in mammalian cells, have pauci-mannose-type N-glycans. In this study, we examined complex-type N-glycans on recombinant proteins via coexpression of human β-1,2-N-acetylglucosaminyltransferase II (hGnT II) and human β1,...

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Detalles Bibliográficos
Autores principales: Kato, Tatsuya, Kako, Natsumi, Kikuta, Kotaro, Miyazaki, Takatsugu, Kondo, Sachiko, Yagi, Hirokazu, Kato, Koichi, Park, Enoch Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431099/
https://www.ncbi.nlm.nih.gov/pubmed/28469195
http://dx.doi.org/10.1038/s41598-017-01630-6