BAMClipper: removing primers from alignments to minimize false-negative mutations in amplicon next-generation sequencing

Amplicon-based next-generation sequencing (NGS) has been widely adopted for genetic variation detection in human and other organisms. Conventional data analysis paradigm includes primer trimming before read mapping. Here we introduce BAMClipper that removes primer sequences after mapping original se...

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Detalles Bibliográficos
Autores principales: Au, Chun Hang, Ho, Dona N., Kwong, Ava, Chan, Tsun Leung, Ma, Edmond S. K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431517/
https://www.ncbi.nlm.nih.gov/pubmed/28484262
http://dx.doi.org/10.1038/s41598-017-01703-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431517/
https://www.ncbi.nlm.nih.gov/pubmed/28484262
http://dx.doi.org/10.1038/s41598-017-01703-6

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