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A genome scan for milk production traits in dairy goats reveals two new mutations in Dgat1 reducing milk fat content
The quantity of milk and milk fat and proteins are particularly important traits in dairy livestock. However, little is known about the regions of the genome that influence these traits in goats. We conducted a genome wide association study in French goats and identified 109 regions associated with...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431851/ https://www.ncbi.nlm.nih.gov/pubmed/28500343 http://dx.doi.org/10.1038/s41598-017-02052-0 |
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author | Martin, Pauline Palhière, Isabelle Maroteau, Cyrielle Bardou, Philippe Canale-Tabet, Kamila Sarry, Julien Woloszyn, Florent Bertrand-Michel, Justine Racke, Ines Besir, Hüseyin Rupp, Rachel Tosser-Klopp, Gwenola |
author_facet | Martin, Pauline Palhière, Isabelle Maroteau, Cyrielle Bardou, Philippe Canale-Tabet, Kamila Sarry, Julien Woloszyn, Florent Bertrand-Michel, Justine Racke, Ines Besir, Hüseyin Rupp, Rachel Tosser-Klopp, Gwenola |
author_sort | Martin, Pauline |
collection | PubMed |
description | The quantity of milk and milk fat and proteins are particularly important traits in dairy livestock. However, little is known about the regions of the genome that influence these traits in goats. We conducted a genome wide association study in French goats and identified 109 regions associated with dairy traits. For a major region on chromosome 14 closely associated with fat content, the Diacylglycerol O-Acyltransferase 1 (DGAT1) gene turned out to be a functional and positional candidate gene. The caprine reference sequence of this gene was completed and 29 polymorphisms were found in the gene sequence, including two novel exonic mutations: R251L and R396W, leading to substitutions in the protein sequence. The R251L mutation was found in the Saanen breed at a frequency of 3.5% and the R396W mutation both in the Saanen and Alpine breeds at a frequencies of 13% and 7% respectively. The R396W mutation explained 46% of the genetic variance of the trait, and the R251L mutation 6%. Both mutations were associated with a notable decrease in milk fat content. Their causality was then demonstrated by a functional test. These results provide new knowledge on the genetic basis of milk synthesis and will help improve the management of the French dairy goat breeding program. |
format | Online Article Text |
id | pubmed-5431851 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-54318512017-05-16 A genome scan for milk production traits in dairy goats reveals two new mutations in Dgat1 reducing milk fat content Martin, Pauline Palhière, Isabelle Maroteau, Cyrielle Bardou, Philippe Canale-Tabet, Kamila Sarry, Julien Woloszyn, Florent Bertrand-Michel, Justine Racke, Ines Besir, Hüseyin Rupp, Rachel Tosser-Klopp, Gwenola Sci Rep Article The quantity of milk and milk fat and proteins are particularly important traits in dairy livestock. However, little is known about the regions of the genome that influence these traits in goats. We conducted a genome wide association study in French goats and identified 109 regions associated with dairy traits. For a major region on chromosome 14 closely associated with fat content, the Diacylglycerol O-Acyltransferase 1 (DGAT1) gene turned out to be a functional and positional candidate gene. The caprine reference sequence of this gene was completed and 29 polymorphisms were found in the gene sequence, including two novel exonic mutations: R251L and R396W, leading to substitutions in the protein sequence. The R251L mutation was found in the Saanen breed at a frequency of 3.5% and the R396W mutation both in the Saanen and Alpine breeds at a frequencies of 13% and 7% respectively. The R396W mutation explained 46% of the genetic variance of the trait, and the R251L mutation 6%. Both mutations were associated with a notable decrease in milk fat content. Their causality was then demonstrated by a functional test. These results provide new knowledge on the genetic basis of milk synthesis and will help improve the management of the French dairy goat breeding program. Nature Publishing Group UK 2017-05-12 /pmc/articles/PMC5431851/ /pubmed/28500343 http://dx.doi.org/10.1038/s41598-017-02052-0 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Martin, Pauline Palhière, Isabelle Maroteau, Cyrielle Bardou, Philippe Canale-Tabet, Kamila Sarry, Julien Woloszyn, Florent Bertrand-Michel, Justine Racke, Ines Besir, Hüseyin Rupp, Rachel Tosser-Klopp, Gwenola A genome scan for milk production traits in dairy goats reveals two new mutations in Dgat1 reducing milk fat content |
title | A genome scan for milk production traits in dairy goats reveals two new mutations in Dgat1 reducing milk fat content |
title_full | A genome scan for milk production traits in dairy goats reveals two new mutations in Dgat1 reducing milk fat content |
title_fullStr | A genome scan for milk production traits in dairy goats reveals two new mutations in Dgat1 reducing milk fat content |
title_full_unstemmed | A genome scan for milk production traits in dairy goats reveals two new mutations in Dgat1 reducing milk fat content |
title_short | A genome scan for milk production traits in dairy goats reveals two new mutations in Dgat1 reducing milk fat content |
title_sort | genome scan for milk production traits in dairy goats reveals two new mutations in dgat1 reducing milk fat content |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431851/ https://www.ncbi.nlm.nih.gov/pubmed/28500343 http://dx.doi.org/10.1038/s41598-017-02052-0 |
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