Cargando…

A genome scan for milk production traits in dairy goats reveals two new mutations in Dgat1 reducing milk fat content

The quantity of milk and milk fat and proteins are particularly important traits in dairy livestock. However, little is known about the regions of the genome that influence these traits in goats. We conducted a genome wide association study in French goats and identified 109 regions associated with...

Descripción completa

Detalles Bibliográficos
Autores principales: Martin, Pauline, Palhière, Isabelle, Maroteau, Cyrielle, Bardou, Philippe, Canale-Tabet, Kamila, Sarry, Julien, Woloszyn, Florent, Bertrand-Michel, Justine, Racke, Ines, Besir, Hüseyin, Rupp, Rachel, Tosser-Klopp, Gwenola
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431851/
https://www.ncbi.nlm.nih.gov/pubmed/28500343
http://dx.doi.org/10.1038/s41598-017-02052-0
_version_ 1783236518723190784
author Martin, Pauline
Palhière, Isabelle
Maroteau, Cyrielle
Bardou, Philippe
Canale-Tabet, Kamila
Sarry, Julien
Woloszyn, Florent
Bertrand-Michel, Justine
Racke, Ines
Besir, Hüseyin
Rupp, Rachel
Tosser-Klopp, Gwenola
author_facet Martin, Pauline
Palhière, Isabelle
Maroteau, Cyrielle
Bardou, Philippe
Canale-Tabet, Kamila
Sarry, Julien
Woloszyn, Florent
Bertrand-Michel, Justine
Racke, Ines
Besir, Hüseyin
Rupp, Rachel
Tosser-Klopp, Gwenola
author_sort Martin, Pauline
collection PubMed
description The quantity of milk and milk fat and proteins are particularly important traits in dairy livestock. However, little is known about the regions of the genome that influence these traits in goats. We conducted a genome wide association study in French goats and identified 109 regions associated with dairy traits. For a major region on chromosome 14 closely associated with fat content, the Diacylglycerol O-Acyltransferase 1 (DGAT1) gene turned out to be a functional and positional candidate gene. The caprine reference sequence of this gene was completed and 29 polymorphisms were found in the gene sequence, including two novel exonic mutations: R251L and R396W, leading to substitutions in the protein sequence. The R251L mutation was found in the Saanen breed at a frequency of 3.5% and the R396W mutation both in the Saanen and Alpine breeds at a frequencies of 13% and 7% respectively. The R396W mutation explained 46% of the genetic variance of the trait, and the R251L mutation 6%. Both mutations were associated with a notable decrease in milk fat content. Their causality was then demonstrated by a functional test. These results provide new knowledge on the genetic basis of milk synthesis and will help improve the management of the French dairy goat breeding program.
format Online
Article
Text
id pubmed-5431851
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-54318512017-05-16 A genome scan for milk production traits in dairy goats reveals two new mutations in Dgat1 reducing milk fat content Martin, Pauline Palhière, Isabelle Maroteau, Cyrielle Bardou, Philippe Canale-Tabet, Kamila Sarry, Julien Woloszyn, Florent Bertrand-Michel, Justine Racke, Ines Besir, Hüseyin Rupp, Rachel Tosser-Klopp, Gwenola Sci Rep Article The quantity of milk and milk fat and proteins are particularly important traits in dairy livestock. However, little is known about the regions of the genome that influence these traits in goats. We conducted a genome wide association study in French goats and identified 109 regions associated with dairy traits. For a major region on chromosome 14 closely associated with fat content, the Diacylglycerol O-Acyltransferase 1 (DGAT1) gene turned out to be a functional and positional candidate gene. The caprine reference sequence of this gene was completed and 29 polymorphisms were found in the gene sequence, including two novel exonic mutations: R251L and R396W, leading to substitutions in the protein sequence. The R251L mutation was found in the Saanen breed at a frequency of 3.5% and the R396W mutation both in the Saanen and Alpine breeds at a frequencies of 13% and 7% respectively. The R396W mutation explained 46% of the genetic variance of the trait, and the R251L mutation 6%. Both mutations were associated with a notable decrease in milk fat content. Their causality was then demonstrated by a functional test. These results provide new knowledge on the genetic basis of milk synthesis and will help improve the management of the French dairy goat breeding program. Nature Publishing Group UK 2017-05-12 /pmc/articles/PMC5431851/ /pubmed/28500343 http://dx.doi.org/10.1038/s41598-017-02052-0 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Martin, Pauline
Palhière, Isabelle
Maroteau, Cyrielle
Bardou, Philippe
Canale-Tabet, Kamila
Sarry, Julien
Woloszyn, Florent
Bertrand-Michel, Justine
Racke, Ines
Besir, Hüseyin
Rupp, Rachel
Tosser-Klopp, Gwenola
A genome scan for milk production traits in dairy goats reveals two new mutations in Dgat1 reducing milk fat content
title A genome scan for milk production traits in dairy goats reveals two new mutations in Dgat1 reducing milk fat content
title_full A genome scan for milk production traits in dairy goats reveals two new mutations in Dgat1 reducing milk fat content
title_fullStr A genome scan for milk production traits in dairy goats reveals two new mutations in Dgat1 reducing milk fat content
title_full_unstemmed A genome scan for milk production traits in dairy goats reveals two new mutations in Dgat1 reducing milk fat content
title_short A genome scan for milk production traits in dairy goats reveals two new mutations in Dgat1 reducing milk fat content
title_sort genome scan for milk production traits in dairy goats reveals two new mutations in dgat1 reducing milk fat content
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431851/
https://www.ncbi.nlm.nih.gov/pubmed/28500343
http://dx.doi.org/10.1038/s41598-017-02052-0
work_keys_str_mv AT martinpauline agenomescanformilkproductiontraitsindairygoatsrevealstwonewmutationsindgat1reducingmilkfatcontent
AT palhiereisabelle agenomescanformilkproductiontraitsindairygoatsrevealstwonewmutationsindgat1reducingmilkfatcontent
AT maroteaucyrielle agenomescanformilkproductiontraitsindairygoatsrevealstwonewmutationsindgat1reducingmilkfatcontent
AT bardouphilippe agenomescanformilkproductiontraitsindairygoatsrevealstwonewmutationsindgat1reducingmilkfatcontent
AT canaletabetkamila agenomescanformilkproductiontraitsindairygoatsrevealstwonewmutationsindgat1reducingmilkfatcontent
AT sarryjulien agenomescanformilkproductiontraitsindairygoatsrevealstwonewmutationsindgat1reducingmilkfatcontent
AT woloszynflorent agenomescanformilkproductiontraitsindairygoatsrevealstwonewmutationsindgat1reducingmilkfatcontent
AT bertrandmicheljustine agenomescanformilkproductiontraitsindairygoatsrevealstwonewmutationsindgat1reducingmilkfatcontent
AT rackeines agenomescanformilkproductiontraitsindairygoatsrevealstwonewmutationsindgat1reducingmilkfatcontent
AT besirhuseyin agenomescanformilkproductiontraitsindairygoatsrevealstwonewmutationsindgat1reducingmilkfatcontent
AT rupprachel agenomescanformilkproductiontraitsindairygoatsrevealstwonewmutationsindgat1reducingmilkfatcontent
AT tosserkloppgwenola agenomescanformilkproductiontraitsindairygoatsrevealstwonewmutationsindgat1reducingmilkfatcontent
AT martinpauline genomescanformilkproductiontraitsindairygoatsrevealstwonewmutationsindgat1reducingmilkfatcontent
AT palhiereisabelle genomescanformilkproductiontraitsindairygoatsrevealstwonewmutationsindgat1reducingmilkfatcontent
AT maroteaucyrielle genomescanformilkproductiontraitsindairygoatsrevealstwonewmutationsindgat1reducingmilkfatcontent
AT bardouphilippe genomescanformilkproductiontraitsindairygoatsrevealstwonewmutationsindgat1reducingmilkfatcontent
AT canaletabetkamila genomescanformilkproductiontraitsindairygoatsrevealstwonewmutationsindgat1reducingmilkfatcontent
AT sarryjulien genomescanformilkproductiontraitsindairygoatsrevealstwonewmutationsindgat1reducingmilkfatcontent
AT woloszynflorent genomescanformilkproductiontraitsindairygoatsrevealstwonewmutationsindgat1reducingmilkfatcontent
AT bertrandmicheljustine genomescanformilkproductiontraitsindairygoatsrevealstwonewmutationsindgat1reducingmilkfatcontent
AT rackeines genomescanformilkproductiontraitsindairygoatsrevealstwonewmutationsindgat1reducingmilkfatcontent
AT besirhuseyin genomescanformilkproductiontraitsindairygoatsrevealstwonewmutationsindgat1reducingmilkfatcontent
AT rupprachel genomescanformilkproductiontraitsindairygoatsrevealstwonewmutationsindgat1reducingmilkfatcontent
AT tosserkloppgwenola genomescanformilkproductiontraitsindairygoatsrevealstwonewmutationsindgat1reducingmilkfatcontent