Identifying conformational changes with site-directed spin labeling reveals that the GTPase domain of HydF is a molecular switch

[FeFe]-hydrogenases catalyse the reduction of protons to hydrogen at a complex 2Fe[4Fe4S] center called H-cluster. The assembly of this active site is a multistep process involving three proteins, HydE, HydF and HydG. According to the current models, HydF has the key double role of scaffold, upon wh...

Descripción completa

Detalles Bibliográficos
Autores principales: Galazzo, Laura, Maso, Lorenzo, De Rosa, Edith, Bortolus, Marco, Doni, Davide, Acquasaliente, Laura, De Filippis, Vincenzo, Costantini, Paola, Carbonera, Donatella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431965/
https://www.ncbi.nlm.nih.gov/pubmed/28490758
http://dx.doi.org/10.1038/s41598-017-01886-y

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431965/
https://www.ncbi.nlm.nih.gov/pubmed/28490758
http://dx.doi.org/10.1038/s41598-017-01886-y

Ejemplares similares