Cargando…

Identifying conformational changes with site-directed spin labeling reveals that the GTPase domain of HydF is a molecular switch

[FeFe]-hydrogenases catalyse the reduction of protons to hydrogen at a complex 2Fe[4Fe4S] center called H-cluster. The assembly of this active site is a multistep process involving three proteins, HydE, HydF and HydG. According to the current models, HydF has the key double role of scaffold, upon wh...

Descripción completa

Detalles Bibliográficos
Autores principales:	Galazzo, Laura, Maso, Lorenzo, De Rosa, Edith, Bortolus, Marco, Doni, Davide, Acquasaliente, Laura, De Filippis, Vincenzo, Costantini, Paola, Carbonera, Donatella
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431965/ https://www.ncbi.nlm.nih.gov/pubmed/28490758 http://dx.doi.org/10.1038/s41598-017-01886-y

Ejemplares similares

Overview of the Maturation Machinery of the H-Cluster of [FeFe]-Hydrogenases with a Focus on HydF
por: Bortolus, Marco, et al.
Publicado: (2018)

Biosynthesis of the catalytic H-cluster of [FeFe] hydrogenase: the roles of the Fe–S maturase proteins HydE, HydF, and HydG
por: Britt, R. David, et al.
Publicado: (2020)

Monitoring H-cluster assembly using a semi-synthetic HydF protein
por: Németh, Brigitta, et al.
Publicado: (2019)

The maturase HydF enables [FeFe] hydrogenase assembly via transient, cofactor-dependent interactions
por: Németh, Brigitta, et al.
Publicado: (2020)

Electron Spin Relaxation and Biochemical Characterization of the Hydrogenase Maturase HydF: Insights into [2Fe-2S] and [4Fe-4S] Cluster Communication and Hydrogenase Activation
por: Shepard, Eric M., et al.
Publicado: (2017)

Effects of Fe(2+)/Fe(3+) Binding to Human Frataxin and Its D122Y Variant, as Revealed by Site-Directed Spin Labeling (SDSL) EPR Complemented by Fluorescence and Circular Dichroism Spectroscopies
por: Doni, Davide, et al.
Publicado: (2020)

A Combined Spectroscopic and In Silico Approach to Evaluate the Interaction of Human Frataxin with Mitochondrial Superoxide Dismutase
por: Doni, Davide, et al.
Publicado: (2021)

Use of Hyd. Potass. in Hydrocephalus
por: Mead, Thompson
Publicado: (1847)

The Role of Proteolysis in Amyloidosis
por: Acquasaliente, Laura, et al.
Publicado: (2022)

Proposed Mechanism for the Biosynthesis of the [FeFe] Hydrogenase H-Cluster: Central Roles for the Radical SAM Enzymes HydG and HydE
por: Britt, R. David, et al.
Publicado: (2021)

Violaxanthin and Zeaxanthin May Replace Lutein at the L1 Site of LHCII, Conserving the Interactions with Surrounding Chlorophylls and the Capability of Triplet–Triplet Energy Transfer
por: Carbonera, Donatella, et al.
Publicado: (2022)

Nucleotide Dependent Switching in Rho GTPase: Conformational Heterogeneity and Competing Molecular Interactions
por: Kumawat, Amit, et al.
Publicado: (2017)

Dual role of a GTPase conformational switch for membrane fusion by mitofusin ubiquitylation
por: Schuster, Ramona, et al.
Publicado: (2019)

A Case of Traumatic Tetanus, Treated with Hyd. Chloral
por: Kinnear, A. H.
Publicado: (1870)

Interplay between conformational selection and zymogen activation
por: Chakraborty, Pradipta, et al.
Publicado: (2018)

HydDB: A web tool for hydrogenase classification and analysis
por: Søndergaard, Dan, et al.
Publicado: (2016)

A Case of Poisoning with Morphia and Hyd. Chloral—Remarkable Recovery
por: Griffin, Theodore
Publicado: (1872)

Tyrosyl phosphorylation of KRAS stalls GTPase cycle via alteration of switch I and II conformation
por: Kano, Yoshihito, et al.
Publicado: (2019)

Cellular Uptake and Antitumor Activity of DOX-hyd-PEG-FA Nanoparticles
por: Ye, Wei-liang, et al.
Publicado: (2014)

The GTPase switch in ribosomal translocation
por: Moore, Pete
Publicado: (2005)

Magnetophotoselection in the Investigation of Excitonically Coupled Chromophores: The Case of the Water-Soluble Chlorophyll Protein
por: Ciuti, Susanna, et al.
Publicado: (2022)

Reversible H Atom Abstraction Catalyzed by the Radical S-Adenosylmethionine Enzyme HydG
por: Duffus, Benjamin R., et al.
Publicado: (2014)

From haemadin to haemanorm: Synthesis and characterization of full‐length haemadin from the leech Haemadipsa sylvestris and of a novel bivalent, highly potent thrombin inhibitor (haemanorm)
por: Acquasaliente, Laura, et al.
Publicado: (2023)

Rab GTPases: Switching to Human Diseases
por: Guadagno, Noemi Antonella, et al.
Publicado: (2019)

Molecular Basis of the Electron Bifurcation Mechanism in the [FeFe]-Hydrogenase Complex HydABC
por: Katsyv, Alexander, et al.
Publicado: (2023)

Nature of the Ligand-Centered Triplet State in Gd(3+) β-Diketonate Complexes as Revealed by Time-Resolved EPR Spectroscopy and DFT Calculations
por: Carlotto, Silvia, et al.
Publicado: (2021)

Rho GTPases in the Amygdala—A Switch for Fears?
por: Sarowar, Tasnuva, et al.
Publicado: (2020)

"Switch to kill, switch to survive" – bacterial toxins modifying Rho GTPases
por: Aktories, K
Publicado: (2009)

Genomic insights into members of the candidate phylum Hyd24-12 common in mesophilic anaerobic digesters
por: Kirkegaard, Rasmus Hansen, et al.
Publicado: (2016)

Von Willebrand Factor Multimers and the Relaxation Response: A One-Year Study
por: Dal Lin, Carlo, et al.
Publicado: (2021)

Hyd ubiquitinates the NF-κB co-factor Akirin to operate an effective immune response in Drosophila
por: Cammarata-Mouchtouris, Alexandre, et al.
Publicado: (2020)

Nucleotide Binding Switches the Information Flow in Ras GTPases
por: Raimondi, Francesco, et al.
Publicado: (2011)

Specific Conformational States of Ras GTPase upon Effector Binding
por: Baussand, Julie, et al.
Publicado: (2012)

Conformal description of spinning particles
por: Todorov, I T
Publicado: (1986)

Synthesis, Conformational Analysis and Antitumor Activity of the Naturally Occurring Antimicrobial Medium-Length Peptaibol Pentadecaibin and Spin-Labeled Analogs Thereof
por: Morbiato, Laura, et al.
Publicado: (2023)

Nucleocapsid assembly in pneumoviruses is regulated by conformational switching of the N protein
por: Renner, Max, et al.
Publicado: (2016)

Potential of the Virion-Associated Peptidoglycan Hydrolase HydH5 and Its Derivative Fusion Proteins in Milk Biopreservation
por: Rodríguez-Rubio, Lorena, et al.
Publicado: (2013)

Arterial Spin Labeling Reveals Disrupted Brain Networks and Functional Connectivity in Drug-Resistant Temporal Epilepsy
por: Boscolo Galazzo, Ilaria, et al.
Publicado: (2019)

In-cell investigation of the conformational landscape of the GTPase UreG by SDSL-EPR
por: Pierro, Annalisa, et al.
Publicado: (2023)

Optical switching of nuclear spin–spin couplings in semiconductors
por: Goto, Atsushi, et al.
Publicado: (2011)

Cannot write session to /tmp/vufind_sessions/sess_u15r14ddlvins7hnfkds92a8pp